caa a22 4500 378904817 CHVBK 20180305123527.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/CCLM.2004.204 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.204 Simplified method for the diameter sizing of serum low-density lipoprotein using polyacrylamide gradient gel electrophoresis [Elektronische Daten] [Hideko Tsukamoto, Izumi Takei, Keiko Ishii, Kiyoaki Watanabe] The appearance of small, dense, low-density lipoprotein in serum has been demonstrated to be associated with increased risk of coronary artery disease. The molecular diameter of low-density lipoprotein is usually measured on the basis of mobility differences on polyacrylamide gel electrophoresis. However, since mobility assessed by this method is seriously affected by the increased levels of serum free fatty acids associated with hypertriglyceridemia, we used polyacrylamide gradient gel electrophoresis to eliminate the interference by fatty acids and devised a simple, precise method of polyacrylamide gradient gel electrophoresis to measure the diameter of small, dense, low-density lipoproteins in serum. We used apoferritin and thyroglobulin, which have a molecular diameter of 12.2 nm and 17.0 nm, respectively, and standard low-density lipoprotein particles having a diameter of 25.7 and 27.0 nm as calibrators, estimated by measurement of negative staining of electron microscopy. We also included apoferritin as an internal standard for polyacrylamide gradient gel electrophoresis. The only stain used was Coomassie brilliant blue, and it was used for lipoprotein staining. When we used low-density lipoprotein of 25.73 nm in diameter as a quality control specimen, the coefficient of variation of the size measurements obtained by our method was less than 1.2%. The new method markedly improved the laboratory procedure for measuring the diameter of low-density lipoproteins. © Walter de Gruyter Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence apoferritin nationallicence Coomassie brilliant blue nationallicence internal standard nationallicence measurement of low-density lipoprotein diameter nationallicence small, dense, low-density lipoprotein nationallicence Tsukamoto Hideko Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut Takei Izumi Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut Ishii Keiko Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut Watanabe Kiyoaki Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/9(2004-09-01), 1009-1012 1434-6621 42:9<1009 2004 42 cclm https://doi.org/10.1515/CCLM.2004.204 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.204 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsukamoto Hideko Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Takei Izumi Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Ishii Keiko Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Watanabe Kiyoaki Department of Laboratory Medicine, Keio University, School of Medicine, Tokyo, Japan aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/9(2004-09-01), 1009-1012 1434-6621 42:9<1009 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter