caa a22 4500 378916106 CHVBK 20180305123553.0 cr unu---uuuuu 161128e20040105xx s 000 0 eng 10.1515/BC.2004.008 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.008 Identification of sialic acids on Leishmania donovani amastigotes [Elektronische Daten] [A. K. Chava, M. Chatterjee, G. J. Gerwig, J. P. Kamerling, C. Mandal] The presence of Neu5Ac on promastigotes of Leishmania donovani, the causative organism of Indian visceral leishmaniasis, has been reported recently. Here we report the occurrence of Neu5Ac as a major component on amastigotes, as well as Neu5Gc, Neu5,9Ac2 and Neu9Ac5Gc as indicated by fluorimetric high performance liquid chromatography and gas liquid chromatography/electron impact mass spectrometry. Furthermore, binding studies with Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), and various Siglecs, showed the presence of both (alpha2-6) and (alpha2-3)linked sialic acids; their binding was reduced after sialidase pretreatment. Western blotting of amastigote membrane glycoproteins with SNA demonstrated the presence of two sialoglycoconjugates of Mr values of 164 000 and 150 000. Similarly, binding of MAA demonstrated the presence of five distinct sialoglycans corresponding to molecular masses of 188, 162, 136, 137 and 124 kDa. Achatinin-H, a lectin that preferentially identifies 9-Oacetylated sialic acid (alpha2-6)linked to GalNAc, demonstrated the occurrence of two 9-Oacetylated sialoglycans with Mr 158 000 and 150 000, and was corroborated by flow cytometry; this binding was abolished by recombinant 9-Oacetylesterase pretreatment. Our results indicate that Neu5Ac w(alpha2-6) and (alpha2-3)linked, as well as Neu5Gc and their 9-Oacetyl derivatives, constitute components of the amastigote cell surface of L. donovani. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Chava A. K. aut Chatterjee M. aut Gerwig G. J. aut Kamerling J. P. aut Mandal C. aut Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 59-66 1431-6730 385:1<59 2004 385 bchm https://doi.org/10.1515/BC.2004.008 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chava A. K. aut NATIONALLICENCE 700 1- Chatterjee M. aut NATIONALLICENCE 700 1- Gerwig G. J. aut NATIONALLICENCE 700 1- Kamerling J. P. aut NATIONALLICENCE 700 1- Mandal C. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 59-66 1431-6730 385:1<59 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter