Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation
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| 008 | 161128e20040105xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1515/BC.2004.010 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.010 | ||
| 245 | 0 | 0 | |a Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation |h [Elektronische Daten] |c [M. C. Ramos, H. Steinbrenner, D. Stuhlmann, H. Sies, P. Brenneisen] |
| 520 | 3 | |a Ultraviolet radiation may cause non-melanoma skin cancer by genetic and epigenetic events. In this study, we investigated in a squamous cell carcinoma cell line, SCL-1, whether UV irradiation modulates the expression of matrix metalloproteinases, known to be involved in tumor progression and metastasis by degradation of extracellular matrix components. UVA or UVB irradiation of SCL-1 resulted in a rapid transcriptional upregulation and increased secretion of two members of the matrix metalloproteinase family, MMP-10 (stromelysin-2) and MMP-1 (interstitial collagenase). The increase in MMP-10 steadystate mRNA levels was detected 1 hour after UVA and 4 h after UVB irradiation, whereas MMP-1 was upregulated 4 h after UVA and 16 h after UVB irradiation of tumor cells. UVinduced phosphorylation of extracellular regulated kinases (ERK-1/2) and p38 stress kinase and increased binding of AP-1 transcription factor preceded the rapid stimulation of MMPs in SCL-1 cells. Incubation of cells with the MEK1/2 inhibitor U0126 or the p38 inhibitor SB202190 abolished the UVA and UVB mediated induction of MMP-1 and MMP-10. In conclusion, this study shows that UV irradiation of squamous cell carcinoma results in a rapid upregulation of MMPs. Our results suggest that the time course of induction of target genes, like MMPs, differs between cell types depending on the stimulus. | |
| 540 | |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a Ramos |D M. C. |4 aut | |
| 700 | 1 | |a Steinbrenner |D H. |4 aut | |
| 700 | 1 | |a Stuhlmann |D D. |4 aut | |
| 700 | 1 | |a Sies |D H. |4 aut | |
| 700 | 1 | |a Brenneisen |D P. |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/1(2004-01-05), 75-86 |x 1431-6730 |q 385:1<75 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.010 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.010 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ramos |D M. C. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Steinbrenner |D H. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stuhlmann |D D. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sies |D H. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Brenneisen |D P. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/1(2004-01-05), 75-86 |x 1431-6730 |q 385:1<75 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||