caa a22 4500 378916130 CHVBK 20180305123553.0 cr unu---uuuuu 161128e20040105xx s 000 0 eng 10.1515/BC.2004.011 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.011 Monitoring human parvovirus B19 virus-like particles and antibody complexes in solution by fluorescence correlation spectroscopy [Elektronische Daten] [J. Toivola, P. O. Michel, L. Gilbert, T. Lahtinen, V. Marjomäki, K. Hedman, M. Vuento, C. Oker-Blom] Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and pastimmunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7x10exp-7 cm(2)s(-1) with an apparent hydrodynamic radius of 14 nm. After incubation of the fluorescent VLPs with an acute phase serum sample, the mobility information obtained from the fluorescence intensity fluctuation by autocorrelation analysis showed an average diffusion coefficient of 1.5x10exp-8 cm(2)s(-1), corresponding to an average radius of 157 nm. In contrast, incubation of the fluorescent VLPs with a pastimmunity serum sample gave an average diffusion coefficient of 3.5x10exp-8 cm(2)s(-1) and a radius of 69 nm. A control serum devoid of B19 antibodies caused a change in the diffusion coefficient from 1.7x10exp-7 to 1.6x10exp-7 cm(2)s(-1), which is much smaller than that observed with acute phase or pastimmunity sera. Thus, VLP-antibody complexes with different diffusion coefficients could be identified for the acute phase and pastimmunity sera. FCS measurement of VLPimmune complexes could be useful in distinguishing between antibodies present in acute phase or past-immunity sera as well as in titration of the VLPs. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Toivola J. aut Michel P. O. aut Gilbert L. aut Lahtinen T. aut Marjomäki V. aut Hedman K. aut Vuento M. aut Oker-Blom C. aut Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 87-93 1431-6730 385:1<87 2004 385 bchm https://doi.org/10.1515/BC.2004.011 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toivola J. aut NATIONALLICENCE 700 1- Michel P. O. aut NATIONALLICENCE 700 1- Gilbert L. aut NATIONALLICENCE 700 1- Lahtinen T. aut NATIONALLICENCE 700 1- Marjomäki V. aut NATIONALLICENCE 700 1- Hedman K. aut NATIONALLICENCE 700 1- Vuento M. aut NATIONALLICENCE 700 1- Oker-Blom C. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 87-93 1431-6730 385:1<87 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter