caa a22 4500 378916165 CHVBK 20180305123553.0 cr unu---uuuuu 161128e20040105xx s 000 0 eng 10.1515/BC.2004.003 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.003 Singlet oxygen-mediated damage to cellular DNA determined by the comet assay associated with DNA repair enzymes [Elektronische Daten] [J.-L. Ravanat, S. Sauvaigo, S. Caillat, G. R. Martinez, M. H. G. Medeiros, P. Di Mascio, A. Favier, J. Cadet] The damage profile produced by the reaction of singlet molecular oxygen with cellular DNA was determined using the comet assay associated with DNA repair enzymes. Singlet oxygen was produced intracellularly by thermal decomposition of a watersoluble endoperoxide of a naphthalene derivative which is able to penetrate through the membrane into mammalian cells. We found that the DNA modifications produced by singlet oxygen were almost exclusively oxidised purines recognised by the formamidopyrimidine DNA N-glycosylase. In contrast, significant amounts of direct strand breaks and alkalilabile sites or oxidised pyrimidines, detectable by the bacterial endonuclease III, were not produced. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Ravanat J.-L aut Sauvaigo S. aut Caillat S. aut Martinez G. R. aut Medeiros M. H. G. aut Mascio P. Di aut Favier A. aut Cadet J. aut Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 17-20 1431-6730 385:1<17 2004 385 bchm https://doi.org/10.1515/BC.2004.003 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ravanat J.-L aut NATIONALLICENCE 700 1- Sauvaigo S. aut NATIONALLICENCE 700 1- Caillat S. aut NATIONALLICENCE 700 1- Martinez G. R. aut NATIONALLICENCE 700 1- Medeiros M. H. G. aut NATIONALLICENCE 700 1- Mascio P. Di aut NATIONALLICENCE 700 1- Favier A. aut NATIONALLICENCE 700 1- Cadet J. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 17-20 1431-6730 385:1<17 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter