caa a22 4500 378916173 CHVBK 20180305123553.0 cr unu---uuuuu 161128e20040105xx s 000 0 eng 10.1515/BC.2004.005 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.005 Structural destabilization of the recombinant thermophilic TthL11 ribosomal protein by a single amino acid substitution [Elektronische Daten] [D. Triantafillidou, E. Persidou, D. Lazarou, P. Andrikopoulos, F. Leontiadou, T. Choli-Papadopoulou] Thermus thermophilus L11 protein has previously been reported to be resistant against tryptic and chymotryptic proteolysis under native conditions. With a single amino acid substitution, namely Trp101Arg, conformational changes were induced that resulted in the exhibition of specific amino acids that served as targets for tryptic and chymotryptic action and rendered the protein highly unstable even during purification. This unexpected process was evidenced by the isolation with size exclusion gel chromatography of the wellstructured chymotryptic N-terminal domain in a high amount and its characterization both by Edman degradation and QTOF-EMS spectroscopy. On the other hand, the substitution of Val38Cys, which did not contribute to structural changes, indicates a very possible implication of this amino acid in the protein methylation process. The data reported in this work illustrate the distinctive amino acid dynamics in a thermophilic protein, which, while serving the function common to its counterparts from mesophilic organisms, has had to adapt to the extreme environmental conditions typical of thermophilic organisms. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Triantafillidou D. aut Persidou E. aut Lazarou D. aut Andrikopoulos P. aut Leontiadou F. aut Choli-Papadopoulou T. aut Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 31-39 1431-6730 385:1<31 2004 385 bchm https://doi.org/10.1515/BC.2004.005 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Triantafillidou D. aut NATIONALLICENCE 700 1- Persidou E. aut NATIONALLICENCE 700 1- Lazarou D. aut NATIONALLICENCE 700 1- Andrikopoulos P. aut NATIONALLICENCE 700 1- Leontiadou F. aut NATIONALLICENCE 700 1- Choli-Papadopoulou T. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 31-39 1431-6730 385:1<31 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter