caa a22 4500 378916181 CHVBK 20180305123553.0 cr unu---uuuuu 161128e20040105xx s 000 0 eng 10.1515/BC.2004.007 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.007 N-linked glycosylation of the human bradykinin B2 receptor is required foroptimal cell-surface expression and coupling [Elektronische Daten] [S. Michineau, L. Muller, A. Pizard, F. Alhenc-Gélas, R. M. Rajerison] To investigate the glycosylation of the human bradykinin B2 receptor and the functional significance of this modification, we studied receptors mutated at single or multiple combinations of the three potential N-linked glycosylation sites, asparagines N3, N12 and N180, in COS-7, HEK 293 and CHOK1 cells. Western blot experiments demonstrated that all three extracellular asparagines are glycosylated. The kinetics of bradykinin binding and receptor sequestration remained unchanged after glycosylation had been suppressed. However, the glycosylated receptors were expressed at the cellsurface to a much greater extent than the nonglycosylated receptor and coupling to phospholipase C was less efficient for receptor lacking N-terminal glycosylation. These results indicate that, for the human bradykinin B2 receptor, glycosylation is not required for optimal ligand binding, but plays an important role in cell-surface addressing and receptor function. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Michineau S. aut Muller L. aut Pizard A. aut Alhenc-Gélas F. aut Rajerison R. M. aut Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 49-57 1431-6730 385:1<49 2004 385 bchm https://doi.org/10.1515/BC.2004.007 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michineau S. aut NATIONALLICENCE 700 1- Muller L. aut NATIONALLICENCE 700 1- Pizard A. aut NATIONALLICENCE 700 1- Alhenc-Gélas F. aut NATIONALLICENCE 700 1- Rajerison R. M. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/1(2004-01-05), 49-57 1431-6730 385:1<49 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter