caa a22 4500 37891684X CHVBK 20180305123555.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.2298/hel0441163e doi (NATIONALLICENCE)gruyter-10.2298/hel0441163e HERITABLE TISSUE CULTURE INDUCED GENETIC VARIATION IN SUNFLOWER (Helianthus annuus L.) AS A TOOL FOR CROP IMPROVEMENT [Elektronische Daten] LA VARIACIÓN GENÉTICA HEREDABLE CREADA POR EL CULTIVO DE TEJIDO COMO MEDIO PARA LA MEJORA GENÉTICA DE GIRASOL (Helianthus annuus L.) / VARIATION GÉNÉTIQUE TRANSMISSIBLE CRÉÉE PAR LA CULTURE DE TISSU EN TANT QUE MOYEN D'AMÉLIORER LE TOURNESOL (Helianthus annuus L.) / [J. Encheva, F. Tsvetkova, P. Ivanov] Immature zygotic embryos from the Bulgarian fertility restorer line R 147 (male component of the commercial hybrid Albena) were used as donor material for induction of direct organogenesis in sunflower (Helianthus annuus L.). Range of spontaneous somaclonal variation among the progenies of regenerants has been assessed. Genetic modifications observed in the regenerants included agronomic traits such as oil content in seed, 1000-seed weight, plant height, petiole length, internode length, number of branches, length of branches, number of ray florets, seed width and seed length. The somaclonal line R 12001 showed a modified architecture and higher oil content in seed than the standard R 147, and very good combining ability. The hybrid Julia, an offspring of the somaclonal line R 12001, showed seed and oil yields higher by 18.3% and 17.0%, respectively, than those in the standards Albena and Super Start. In the 2002 tests of the State Variety Testing Commission, the hybrid Julia had a higher seed yield than the standards's mean. Such combination of these favorable changes is desirable in breeding work on sunflower. Somaclonal variation through direct organogenesis facilitated the creation of genetically heritable variation in sunflower, which can be used with for effective production of highly productive and adaptable hybrids. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. KeywordsHelianthus annuus L nationallicence direct organogenesis nationallicence somaclonal variation nationallicence new breeding material nationallicence combining ability nationallicence Encheva J. Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria aut Tsvetkova F. Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria aut Ivanov P. Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria aut HELIA De Gruyter 27/41(2004-12-01), 163-172 1018-1806 27:41<163 2004 27 helia https://doi.org/10.2298/hel0441163e text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0441163e text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Encheva J. Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria aut NATIONALLICENCE 700 1- Tsvetkova F. Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria aut NATIONALLICENCE 700 1- Ivanov P. Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/41(2004-12-01), 163-172 1018-1806 27:41<163 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter