caa a22 4500 378916939 CHVBK 20180305123555.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.2298/hel0441143p doi (NATIONALLICENCE)gruyter-10.2298/hel0441143p BIOCHEMICAL CHARACTERISTICS AND NUTRIENT CONTENT OF THE CALLUS OF SUNFLOWER INBRED LINES [Elektronische Daten] CARACTERÍSTICAS BIOQUÍMICAS Y EL CONTENIDO DE NUTRIENTES EN LOS CALLOS DE LAS LÍNEAS CONSANGUÍNEAS (INBRED) DE GIRASOL / CARACTÉRISTIQUES BIOCHIMIQUES ET CONTENU DE NUTRIMENT DANS LES CALLUS DES LIGNÉES DE TOURNESOL / [Slobodanka Pajević, Dragana Vasić, Petar Sekulić] Mineral nutrition is a factor affecting in vitro regeneration. Various requirements for individual mineral elements between plant species and also between genotypes of the same species have led to the differentiation and definition of the significance of the genetic background in determining callus biochemical characteristics and a confirmation whether different requirements for nutrients leave a possibility of achieving better and more efficient regeneration with a specific modification of substratum composition. The practical aspect of the obtained results lays in the fact that the sunflower species are characterized by low regeneration ability. It was shown that the dynamics of uptake and accumulation of mineral elements into callus cells is a genotype dependent trait being more or less evident depending upon element. Total nitrogen content was between 4 and 5%. The sunflower genotype PH-BC2-101A showed the highest accumulation of nitrogen, phosphorus and, to some extent, calcium. Consequently, a high accumulation of total dry matter without pigment synthesis was recorded. A high nitrogen accumulation in the cited genotype resulting in the highest nitrate reductase activity a very high accumulation of soluble proteins (enzymes). This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. sunflower inbreds nationallicence calluses nationallicence mineral nutrition nationallicence soluble proteins nationallicence pigments nationallicence nutrients uptake nationallicence Pajević Slobodanka Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovića 2, 21000 Novi Sad, Serbia and Montenegro aut Vasić Dragana Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro aut Sekulić Petar Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro aut HELIA De Gruyter 27/41(2004-12-01), 143-150 1018-1806 27:41<143 2004 27 helia https://doi.org/10.2298/hel0441143p text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0441143p text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pajević Slobodanka Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovića 2, 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 700 1- Vasić Dragana Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 700 1- Sekulić Petar Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/41(2004-12-01), 143-150 1018-1806 27:41<143 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter