caa a22 4500 378916963 CHVBK 20180305123555.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.2298/hel0441137v doi (NATIONALLICENCE)gruyter-10.2298/hel0441137v METHODS OF INOCULATION OF SUNFLOWER HEADS WITH Sclerotinia sclerotiorum [Elektronische Daten] MÉTODOS DE INOCULACIÓN DEL CAPÍTULO DE GIRASOL CON Sclerotinia sclerotiorum / MÉTHODES D'INOCULATION DES CAPITULE DE TOURNESOL AVEC Sclerotinia sclerotiorum / [G. Van Becelaere, J.F. Miller] Sclerotinia head rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease in sunflower (Helianthus annuus L.). Development of hybrids with adequate genetic resistance is necessary to reduce yield losses. The objective of this study was to find an effective technique of inoculating sunflower heads with Sclerotinia sclerotiorum for screening and identifying resistant and susceptible genotypes. A factorial experiment was conducted to evaluate the effectiveness of different inoculation procedures. The factors were bag color, water treatment, inoculum type, and rate of inoculation. Based on the results of this study, the inoculation procedure recommended to sunflower researchers who wish to screen lines or hybrids for Sclerotinia head rot resistance is spraying the heads at the beginning of flowering with 5 cm 3 of a suspension of ascospores containing 5000 ascospores per milliliter and covering the heads with brown paper bags immediately after inoculation. Application of additional water is not needed. Measurements of infection could begin as early as 35 days after inoculation. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Sclerotinia sclerotiorum nationallicence head rot nationallicence sunflower nationallicence disease resistance nationallicence Van Becelaere G. North Dakota State University, Plant Sciences Department, Loftsgard Hall, Fargo, ND, USA, Phone: (701) 239-1321 aut Miller J.F. USDA-ARS, Northern Crop Science Laboratory, PO Box 5677, Fargo, ND, USA aut HELIA De Gruyter 27/41(2004-12-01), 137-142 1018-1806 27:41<137 2004 27 helia https://doi.org/10.2298/hel0441137v text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0441137v text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Becelaere G. North Dakota State University, Plant Sciences Department, Loftsgard Hall, Fargo, ND, USA, Phone: (701) 239-1321 aut NATIONALLICENCE 700 1- Miller J.F. USDA-ARS, Northern Crop Science Laboratory, PO Box 5677, Fargo, ND, USA aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/41(2004-12-01), 137-142 1018-1806 27:41<137 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter