caa a22 4500 378916971 CHVBK 20180305123555.0 cr unu---uuuuu 161128e20041201xx s 000 0 eng 10.2298/hel0441041d doi (NATIONALLICENCE)gruyter-10.2298/hel0441041d MUTATION BREEDING IN SUNFLOWER FOR RESISTANCE TO ALTERNARIA LEAF SPOT [Elektronische Daten] MUTACIÓN PARA MEJORAR GIRASOL CON RESISTENCIA A LA ALTERNARIOSIS DE LA HOJA / MUTATION POUR L'AMELIORATION DE LA RESISTANCE DU TOURNESOL VIS-A-VIS DE LA ROUILLE DES FEUILLES / [Marcelo F. de Oliveira, Augusto Tulmann Neto, Regina M.V.B.C. Leite, Vania B.R. Castiglioni, C.A.A. Arias] Genetic variability for resistance to Alternaria leaf spot disease (Alternariahelianthi) can be induced by radiation or chemical mutagens. The objectives of this study were to create genetic variability in cultivated sunflower and to select lines resistant to Alternaria leaf spot. In the first experiment, sunflower seeds of the genotype HA BR 104 were irradiated with 150 and 165 Gy of gamma rays. Seeds were sown in the field at the Embrapa Soybean experimental station, in Londrina, PR, Brazil and M1 plants were harvested in bulk. M2 ,M3 and M4 plants were screened for disease resistance under natural infection in the field. Plants were evaluated for Alternaria leaf spot symptoms, using a diagrammatic scale from 0 (no symptoms) to 5 (maximum infection). Before flowering, plants showing no symptoms of Alternaria leaf spot (grade 0) or less than 5% diseased leaf area (grade 1) were bagged for self-pollination. Self-pollinated plants and open-pollinated plants from 150 Gy and 165 Gy populations with no or mild disease symptoms were selected. In the second experiment, sunflower seeds of the genotypes HA 300 and HA BR 104 were treated with ethyl methanesulfonate (EMS) at 0.015 mol dm-3. Selected M2 and M3 were screened for disease resistance in the field. From the EMS treatment, 300 M3 plants with no disease were recovered. All these lines will be tested for combining ability. The best lines will be used for hybrid production. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Helianthus annuus nationallicence Alternaria helianthi nationallicence gamma ray nationallicence chemical mutagen nationallicence Brazil nationallicence Oliveira Marcelo F. de Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil, Phone: 00 55 43 3371-6000, Fax: 00 55 43 3371-6100 aut Neto Augusto Tulmann Agricultural Nuclear Energy Center (CENA-USP), Piracicaba, SP, Brazil aut Leite Regina M.V.B.C. Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil aut Castiglioni Vania B.R. Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil aut Arias C.A.A. Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil aut HELIA De Gruyter 27/41(2004-12-01), 41-50 1018-1806 27:41<41 2004 27 helia https://doi.org/10.2298/hel0441041d text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.2298/hel0441041d text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oliveira Marcelo F. de Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil, Phone: 00 55 43 3371-6000, Fax: 00 55 43 3371-6100 aut NATIONALLICENCE 700 1- Neto Augusto Tulmann Agricultural Nuclear Energy Center (CENA-USP), Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- Leite Regina M.V.B.C. Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil aut NATIONALLICENCE 700 1- Castiglioni Vania B.R. Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil aut NATIONALLICENCE 700 1- Arias C.A.A. Embrapa Soybean, Caixa Postal 231, 86001-970, Londrina, PR, Brazil aut NATIONALLICENCE 773 0- HELIA De Gruyter 27/41(2004-12-01), 41-50 1018-1806 27:41<41 2004 27 helia CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter