Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood
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| 024 | 7 | 0 | |a 10.1515/JPM.2004.144 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/JPM.2004.144 | ||
| 245 | 0 | 0 | |a Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood |h [Elektronische Daten] |c [Corrina Powell, Nicolas Orsi, Nigel Simpson, Malcolm Levene] |
| 520 | 3 | |a Objective: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood. Methods: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, interferon (IFN)-γ and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean ± S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test. Results: All cytokine capacities increased rapidly by 1 hour (p < 0.001), except IL-10 (p = 0.04). TNF-α peaked between 3-6 hours (1581 ± 377 pg/million WC), declining by 24 hours. Similarly, IFN-γ peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 ± 75 pg/million WC) and IL-10 (311 ± 37 pg/million WC). Overall, IFN-γ capacity was lowest (72 ± 10 pg/million WC) and IL-6 capacity was greatest (61489 ± 7059 pg/million WC). Conclusion: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-γ production. These results may expand our knowledge of neonatal disease, etiology and management. | |
| 540 | |a © Walter de Gruyter | ||
| 690 | 7 | |a Human reproduction, growth & development |2 nationallicence | |
| 690 | 7 | |a Gynaecology & obstetrics |2 nationallicence | |
| 690 | 7 | |a Paediatric medicine |2 nationallicence | |
| 690 | 7 | |a Cytokines |2 nationallicence | |
| 690 | 7 | |a term neonate |2 nationallicence | |
| 690 | 7 | |a inflammation |2 nationallicence | |
| 690 | 7 | |a lipopolysaccharide |2 nationallicence | |
| 690 | 7 | |a stimulation |2 nationallicence | |
| 700 | 1 | |a Powell |D Corrina |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. |4 aut | |
| 700 | 1 | |a Orsi |D Nicolas |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. |4 aut | |
| 700 | 1 | |a Simpson |D Nigel |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. |4 aut | |
| 700 | 1 | |a Levene |D Malcolm |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. |4 aut | |
| 773 | 0 | |t Journal of Perinatal Medicine |d Walter de Gruyter |g 32/5(2004-09-01), 440-445 |x 0300-5577 |q 32:5<440 |1 2004 |2 32 |o jpme | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/JPM.2004.144 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/JPM.2004.144 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Powell |D Corrina |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Orsi |D Nicolas |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Simpson |D Nigel |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Levene |D Malcolm |u Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Perinatal Medicine |d Walter de Gruyter |g 32/5(2004-09-01), 440-445 |x 0300-5577 |q 32:5<440 |1 2004 |2 32 |o jpme | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||