caa a22 4500 378917196 CHVBK 20180305123556.0 cr unu---uuuuu 161128e20040901xx s 000 0 eng 10.1515/JPM.2004.144 doi (NATIONALLICENCE)gruyter-10.1515/JPM.2004.144 Characterisation of the cytokine inflammatory response in LPS stimulated full-term cord blood [Elektronische Daten] [Corrina Powell, Nicolas Orsi, Nigel Simpson, Malcolm Levene] Objective: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood. Methods: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, interferon (IFN)-γ and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean ± S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test. Results: All cytokine capacities increased rapidly by 1 hour (p < 0.001), except IL-10 (p = 0.04). TNF-α peaked between 3-6 hours (1581 ± 377 pg/million WC), declining by 24 hours. Similarly, IFN-γ peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 ± 75 pg/million WC) and IL-10 (311 ± 37 pg/million WC). Overall, IFN-γ capacity was lowest (72 ± 10 pg/million WC) and IL-6 capacity was greatest (61489 ± 7059 pg/million WC). Conclusion: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-γ production. These results may expand our knowledge of neonatal disease, etiology and management. © Walter de Gruyter Human reproduction, growth & development nationallicence Gynaecology & obstetrics nationallicence Paediatric medicine nationallicence Cytokines nationallicence term neonate nationallicence inflammation nationallicence lipopolysaccharide nationallicence stimulation nationallicence Powell Corrina Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. aut Orsi Nicolas Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. aut Simpson Nigel Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. aut Levene Malcolm Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K. aut Journal of Perinatal Medicine Walter de Gruyter 32/5(2004-09-01), 440-445 0300-5577 32:5<440 2004 32 jpme https://doi.org/10.1515/JPM.2004.144 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/JPM.2004.144 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Powell Corrina Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K aut NATIONALLICENCE 700 1- Orsi Nicolas Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K aut NATIONALLICENCE 700 1- Simpson Nigel Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K aut NATIONALLICENCE 700 1- Levene Malcolm Academic Unit of Pediatrics, Obstetrics and Gynecology, University of Leeds School of Medicine, Leeds General Infirmary, Leeds, U.K aut NATIONALLICENCE 773 0- Journal of Perinatal Medicine Walter de Gruyter 32/5(2004-09-01), 440-445 0300-5577 32:5<440 2004 32 jpme CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter