The effect of processing and cryopreservation on nucleated umbilical cord blood cells
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| 024 | 7 | 0 | |a 10.1515/JPM.2004.142 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/JPM.2004.142 | ||
| 245 | 0 | 4 | |a The effect of processing and cryopreservation on nucleated umbilical cord blood cells |h [Elektronische Daten] |c [Lucy A. Bayer-Zwirello, Despina E. Hoffman, Lorrie A. Adams, Paul T. Wilder, Margaret T. Reece] |
| 520 | 3 | |a Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque™ or Puregene® red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 ± 10% (average ± standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 ± 20% compared to 91 ± 11% (p < 0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen. | |
| 540 | |a © Walter de Gruyter | ||
| 690 | 7 | |a Human reproduction, growth & development |2 nationallicence | |
| 690 | 7 | |a Gynaecology & obstetrics |2 nationallicence | |
| 690 | 7 | |a Paediatric medicine |2 nationallicence | |
| 690 | 7 | |a Blood |2 nationallicence | |
| 690 | 7 | |a cells |2 nationallicence | |
| 690 | 7 | |a cryopreservation |2 nationallicence | |
| 690 | 7 | |a nucleated |2 nationallicence | |
| 690 | 7 | |a recovery |2 nationallicence | |
| 690 | 7 | |a umbilical cord blood |2 nationallicence | |
| 700 | 1 | |a Bayer-Zwirello |D Lucy A. |u Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth's Medical Center, Boston, Tufts University School of Medicine, Boston, Massachusetts, U.S.A. |4 aut | |
| 700 | 1 | |a Hoffman |D Despina E. |u Women's Health Research Laboratory, Baystate Medical Center, Springfield, U.S.A. |4 aut | |
| 700 | 1 | |a Adams |D Lorrie A. |u Department of Microbiology, University of Massachusetts, Amherst, U.S.A. |4 aut | |
| 700 | 1 | |a Wilder |D Paul T. |4 aut | |
| 700 | 1 | |a Reece |D Margaret T. |u Women's Health Research Laboratory, Baystate Medical Center, Springfield, U.S.A. |4 aut | |
| 773 | 0 | |t Journal of Perinatal Medicine |d Walter de Gruyter |g 32/5(2004-09-01), 430-433 |x 0300-5577 |q 32:5<430 |1 2004 |2 32 |o jpme | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/JPM.2004.142 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/JPM.2004.142 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bayer-Zwirello |D Lucy A. |u Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth's Medical Center, Boston, Tufts University School of Medicine, Boston, Massachusetts, U.S.A |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hoffman |D Despina E. |u Women's Health Research Laboratory, Baystate Medical Center, Springfield, U.S.A |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Adams |D Lorrie A. |u Department of Microbiology, University of Massachusetts, Amherst, U.S.A |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wilder |D Paul T. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Reece |D Margaret T. |u Women's Health Research Laboratory, Baystate Medical Center, Springfield, U.S.A |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Perinatal Medicine |d Walter de Gruyter |g 32/5(2004-09-01), 430-433 |x 0300-5577 |q 32:5<430 |1 2004 |2 32 |o jpme | ||
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| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||