caa a22 4500 378917498 CHVBK 20180305123557.0 cr unu---uuuuu 161128e20041101xx s 000 0 eng 10.1515/CCLM.2004.252 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2004.252 Analysis of γ-hydroxybutyric acid, DL-lactic acid, glycolic acid, ethylene glycol and other glycols in body fluids by a direct injection gas chromatography-mass spectrometry assay for wide use [Elektronische Daten] [Paul Van hee, Hugo Neels, Mireille De Doncker, Nicolas Vrydags, Katinka Schatteman, Wim Uyttenbroeck, Nicole Hamers, Dirk Himpe, Willy Lambert] Analysis of blood of severely intoxicated patients always requires prompt investigation. Diagnosis of intoxication with ethylene glycol, γ-hydroxybutyric acid or D-lactic acid takes hours, since several different procedures are required. Rapid derivatization of the common hydroxyl function may resolve this analytical problem. Here we describe a fast method for the simultaneous measurement of ethylene glycol, glycolic acid, γ-hydroxybutyric acid and racemic lactic acid. Only 20 µl of serum, plasma or urine are required for immediate derivatization at 70°C with 750 µl of bis-N,O-trimethylsilyl trifluoroacetamide after adding 20 µl of internal standard solution (1,3-propylene glycol) and 20 µl of the catalyst dimethylformamide. After centrifugation an aliquot is transferred to a gas chromatographic system and analyzed with electron-impact mass spectrometry in selective ion monitoring mode. The derivatized acids and ethylene glycol are well separated and detected with a limit of detection ranging from 0.12 mg/l for ethylene glycol to 0.95 mg/l for γ-hydroxybutyric acid, while the limit of quantification ranged from 0.4 mg/l for ethylene glycol to 3.15 mg/l for γ-hydroxybutyric acid. The method is linear from 0.5 to 1800 mg/l blood for ethylene glycol, from 0.7 to 1200 mg/l for lactic acid, from 1.2 to 1800 mg/l for glycolic acid, and from 3.2 to 200 mg/l for γ-hydroxybutyric acid, with analytical recoveries, accuracy, day-to-day and within-day precision well within the required limits. Total analysis time with one calibrator was 30 min, derivatization time included. This method is very suitable for emergency toxicology, since several toxic substances can be quantified simultaneously in a fast and sensitive manner. © Walter de Gruyter Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence bis-N,O-trimethylsilyl trifluoroacetamide (BSTFA) derivatization nationallicence gas chromatography-mass spectrometry nationallicence glycols nationallicence hydroxy acids nationallicence Van hee Paul Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut Neels Hugo Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut De Doncker Mireille Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut Vrydags Nicolas Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut Schatteman Katinka Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut Uyttenbroeck Wim Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut Hamers Nicole Institute of Public Health Louis Pasteur, Brussels, Belgium aut Himpe Dirk Department of Anesthesiology, Ziekenhuis Netwerk Antwerpen Middelheim, Antwerp, Belgium aut Lambert Willy Laboratory of Toxicology, Ghent University, Ghent, Belgium aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/11(2004-11-01), 1341-1345 1434-6621 42:11<1341 2004 42 cclm https://doi.org/10.1515/CCLM.2004.252 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2004.252 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van hee Paul Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut NATIONALLICENCE 700 1- Neels Hugo Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut NATIONALLICENCE 700 1- De Doncker Mireille Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut NATIONALLICENCE 700 1- Vrydags Nicolas Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut NATIONALLICENCE 700 1- Schatteman Katinka Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut NATIONALLICENCE 700 1- Uyttenbroeck Wim Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium aut NATIONALLICENCE 700 1- Hamers Nicole Institute of Public Health Louis Pasteur, Brussels, Belgium aut NATIONALLICENCE 700 1- Himpe Dirk Department of Anesthesiology, Ziekenhuis Netwerk Antwerpen Middelheim, Antwerp, Belgium aut NATIONALLICENCE 700 1- Lambert Willy Laboratory of Toxicology, Ghent University, Ghent, Belgium aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 42/11(2004-11-01), 1341-1345 1434-6621 42:11<1341 2004 42 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter