Enzymatic U(VI) reduction by Desulfosporosinus species

Verfasser / Beitragende:
[Yohey Suzuki, Shelly D. Kelly, Ken M. Kemner, Jillian F. Banfield]
Ort, Verlag, Jahr:
2004
Enthalten in:
Radiochimica Acta/International journal for chemical aspects of nuclear science and technology, 92/1/2004(2004-01-01), 11-16
Format:
Artikel (online)
ID: 378917757
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024 7 0 |a 10.1524/ract.92.1.11.25404  |2 doi 
035 |a (NATIONALLICENCE)gruyter-10.1524/ract.92.1.11.25404 
245 0 0 |a Enzymatic U(VI) reduction by Desulfosporosinus species  |h [Elektronische Daten]  |c [Yohey Suzuki, Shelly D. Kelly, Ken M. Kemner, Jillian F. Banfield] 
520 3 |a Here we tested U(VI) reduction by a Desulfosporosinus species (sp.) isolate and type strain (DSM 765) in cell suspensions (pH 7) containing 1mM U(VI) and lactate, under an atmosphere containing N2-CO2-H2 (90:5:5). Although neither Desulfosporosinus species (spp.) reduced U(VI) in cell suspensions with 0.25 Na-bicarbonate or 0.85 NaCl, U(VI) was reduced in these solutions by a control strain, Desulfovibrio desulfuricans (ATCC 642). However, both Desulfosporosinus strains reduced U(VI) in cell suspensions depleted in bicarbonate and NaCl. No U(VI) reduction was observed without lactate and H2 electron donors or with heat-killed cells, indicating enzymatic U(VI) reduction. Uranium(VI) reduction by both strains was inhibited when 1mM CuCl2 was added to the cell suspensions. Because the Desulfosporosinus DSM 765 does not contain cytochrome c3 used by Desulfovibrio spp. to reduce U(VI), Desulfosporosinus species reduce uranium via a different enzymatic pathway. 
540 |a © 2004 Oldenbourg Wissenschaftsverlag GmbH 
690 7 |a Laboratory techniques, experiments  |2 nationallicence 
690 7 |a Analytical chemistry  |2 nationallicence 
690 7 |a Inorganic chemistry  |2 nationallicence 
700 1 |a Suzuki  |D Yohey  |4 aut 
700 1 |a Kelly  |D Shelly D.  |4 aut 
700 1 |a Kemner  |D Ken M.  |4 aut 
700 1 |a Banfield  |D Jillian F.  |4 aut 
773 0 |t Radiochimica Acta/International journal for chemical aspects of nuclear science and technology  |d Oldenbourg Wissenschaftsverlag GmbH  |g 92/1/2004(2004-01-01), 11-16  |x 0033-8230  |q 92:1/2004<11  |1 2004  |2 92  |o ract 
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950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kelly  |D Shelly D.  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kemner  |D Ken M.  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Banfield  |D Jillian F.  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Radiochimica Acta/International journal for chemical aspects of nuclear science and technology  |d Oldenbourg Wissenschaftsverlag GmbH  |g 92/1/2004(2004-01-01), 11-16  |x 0033-8230  |q 92:1/2004<11  |1 2004  |2 92  |o ract 
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949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-gruyter