caa a22 4500 378917757 CHVBK 20180305123557.0 cr unu---uuuuu 161128e20040101xx s 000 0 eng 10.1524/ract.92.1.11.25404 doi (NATIONALLICENCE)gruyter-10.1524/ract.92.1.11.25404 Enzymatic U(VI) reduction by Desulfosporosinus species [Elektronische Daten] [Yohey Suzuki, Shelly D. Kelly, Ken M. Kemner, Jillian F. Banfield] Here we tested U(VI) reduction by a Desulfosporosinus species (sp.) isolate and type strain (DSM 765) in cell suspensions (pH 7) containing 1mM U(VI) and lactate, under an atmosphere containing N2-CO2-H2 (90:5:5). Although neither Desulfosporosinus species (spp.) reduced U(VI) in cell suspensions with 0.25 Na-bicarbonate or 0.85 NaCl, U(VI) was reduced in these solutions by a control strain, Desulfovibrio desulfuricans (ATCC 642). However, both Desulfosporosinus strains reduced U(VI) in cell suspensions depleted in bicarbonate and NaCl. No U(VI) reduction was observed without lactate and H2 electron donors or with heat-killed cells, indicating enzymatic U(VI) reduction. Uranium(VI) reduction by both strains was inhibited when 1mM CuCl2 was added to the cell suspensions. Because the Desulfosporosinus DSM 765 does not contain cytochrome c3 used by Desulfovibrio spp. to reduce U(VI), Desulfosporosinus species reduce uranium via a different enzymatic pathway. © 2004 Oldenbourg Wissenschaftsverlag GmbH Laboratory techniques, experiments nationallicence Analytical chemistry nationallicence Inorganic chemistry nationallicence Suzuki Yohey aut Kelly Shelly D. aut Kemner Ken M. aut Banfield Jillian F. aut Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 92/1/2004(2004-01-01), 11-16 0033-8230 92:1/2004<11 2004 92 ract https://doi.org/10.1524/ract.92.1.11.25404 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1524/ract.92.1.11.25404 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki Yohey aut NATIONALLICENCE 700 1- Kelly Shelly D. aut NATIONALLICENCE 700 1- Kemner Ken M. aut NATIONALLICENCE 700 1- Banfield Jillian F. aut NATIONALLICENCE 773 0- Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 92/1/2004(2004-01-01), 11-16 0033-8230 92:1/2004<11 2004 92 ract CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter