caa a22 4500 378919962 CHVBK 20180305123602.0 cr unu---uuuuu 161128e20040413xx s 000 0 eng 10.1515/BC.2004.028 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.028 Inhibition of lentil copper/TPQ amine oxidase by the mechanism-based inhibitor derived from tyramine [Elektronische Daten] [A. Padiglia, G. Floris, S. Longu, M. E. Schininà, J. Z. Pedersen, A. Finazzi Agrò, F. De Angelis, R. Medda] Copper amine oxidase from lentil (Lens esculenta) seedlings was shown to catalyze the oxidative deamination of tyramine and three similar aromatic monoamines, benzylamine, phenylethylamine and 4-methoxyphenylethylamine. Tyramine, an important plant intermediate, was found to be both a substrate and an irreversible inhibitor of the enzyme whereas the other amines were not inhibitory. In the course of tyramine oxidation the enzyme gradually became inactivated with the concomitant appearance of a new absorption at 560 nm due to the formation of a stable adduct. Inactivation took place only in the presence of oxygen and was probably due to the reaction of the enzyme with the oxidation product of tyramine, phydroxyphenylacetaldehyde. The kinetic data obtained in this study indicate that tyramine represents a new interesting type of physiological mechanismbased inhibitor for plant copper amine oxidases. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Padiglia A. aut Floris G. aut Longu S. aut Schininà M. E. aut Pedersen J. Z. aut Agrò A. Finazzi aut Angelis F. De aut Medda R. aut Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 323-329 1431-6730 385:3-4<323 2004 385 bchm https://doi.org/10.1515/BC.2004.028 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.028 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Padiglia A. aut NATIONALLICENCE 700 1- Floris G. aut NATIONALLICENCE 700 1- Longu S. aut NATIONALLICENCE 700 1- Schininà M. E. aut NATIONALLICENCE 700 1- Pedersen J. Z. aut NATIONALLICENCE 700 1- Agrò A. Finazzi aut NATIONALLICENCE 700 1- Angelis F. De aut NATIONALLICENCE 700 1- Medda R. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 323-329 1431-6730 385:3-4<323 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter