caa a22 4500 378920030 CHVBK 20180305123603.0 cr unu---uuuuu 161128e20040413xx s 000 0 eng 10.1515/BC.2004.030 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.030 Critical O2 and NO concentrations in NO-induced cell death in a rat liver sinusoidalendothelial cell line [Elektronische Daten] [N. Dehne, T. Li, F. Petrat, U. Rauen, H. de Groot] Nitric oxide (NO) plus oxygen (O2) are known to cause cell damage via formation of reactive nitrogen species. NO itself directly inhibits cytochrome oxidase of the mitochondrial respiratory chain in competition with O2, thus inducing a hypoxic-like injury. To assess the critical NO and O2 concentrations for both mechanisms of NO-induced cell injury, cells of a rat liver sinusoidal endothelial cell line were incubated in the presence of the NO donor spermineNONOate at different O2 concentrations, and their loss of viability was determined by the release of lactate dehydrogenase. Protection by ascorbic acid was used as indication for the involvement of reactive nitrogen species, whereas a hypoxiclike injury was indicated by the protective effects of glycine and glucose and the increase in NAD(P)H fluorescence. High concentrations of NO (approx. 10 uM NO) and O2 (21% O2) were required to induce endothelial cell death mediated by formation of reactive nitrogen species. On the other hand, pathophysiologically relevant NO concentrations at low but physiological O2 concentrations (ca. 2 uM NO at 5% O2 and about 1 uM NO at 2% O2) induced hypoxiclike cell death in the endothelial cells that was prevented by the presence of glucose. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Dehne N. aut Li T. aut Petrat F. aut Rauen U. aut Groot H. de aut Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 341-349 1431-6730 385:3-4<341 2004 385 bchm https://doi.org/10.1515/BC.2004.030 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dehne N. aut NATIONALLICENCE 700 1- Li T. aut NATIONALLICENCE 700 1- Petrat F. aut NATIONALLICENCE 700 1- Rauen U. aut NATIONALLICENCE 700 1- Groot H. de aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 341-349 1431-6730 385:3-4<341 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter