caa a22 4500 378920049 CHVBK 20180305123603.0 cr unu---uuuuu 161128e20040413xx s 000 0 eng 10.1515/BC.2004.017 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.017 Visualization of the three-dimensional organization of hypoxia-inducible factor-1α and interacting cofactors in subnuclear structures [Elektronische Daten] [U. Berchner-Pfannschmidt, C. Wotzlaw, E. Merten, H. Acker, J. Fandrey] Cells need oxygen (O2) to meet their metabolic demands. Highly efficient systems of O2-sensing have evolved to initiate responses enabling cells to adapt their metabolism to reduced O2 availability. Of central importance is the activation of hypoxiainducible factor-1 (HIF-1), a transcription factor complex that controls the expression of genes the products of which regulate glucose uptake and metabolism, vasotonus and angiogenesis, oxygen capacity of the blood as well as cell growth and death. Activation of HIF-1 requires the accumulation and nuclear translocation of the HIF-1α subunit, its dimerization with HIF-1β and the binding of coactivator proteins such as p300. In this study we investigated the threedimensional (3D) distribution of HIF-1α within the nucleus and assigned its localization to known nuclear compartments. Using twophoton microscopy we determined the colocalization of HIF-1α and -β subunits within nuclear domains as well as overlaps between HIF-1α and p300. Our data provide information on the nuclear distribution of HIF-1α with respect to subnuclear domains that could serve as specific locations for hypoxiainduced gene expression. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Berchner-Pfannschmidt U. aut Wotzlaw C. aut Merten E. aut Acker H. aut Fandrey J. aut Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 231-237 1431-6730 385:3-4<231 2004 385 bchm https://doi.org/10.1515/BC.2004.017 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.017 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berchner-Pfannschmidt U. aut NATIONALLICENCE 700 1- Wotzlaw C. aut NATIONALLICENCE 700 1- Merten E. aut NATIONALLICENCE 700 1- Acker H. aut NATIONALLICENCE 700 1- Fandrey J. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 231-237 1431-6730 385:3-4<231 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter