caa a22 4500 37892009X CHVBK 20180305123603.0 cr unu---uuuuu 161128e20040413xx s 000 0 eng 10.1515/BC.2004.029 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.029 Enhanced expression of basolateral multidrug resistance protein isoforms Mrp3 and Mrp5 in rat liver by LPS [Elektronische Daten] [M. G. Donner, U. Warskulat, N. Saha, D. Häussinger] Lipopolysaccharide (LPS) induces hepatocellular downregulation and endocytic retrieval of multidrug resistance protein 2 (Mrp2, Abcc2). Basolateral Mrp isoforms may compensate for the intracellular metabolic changes in cholestasis. Therefore, the effect of LPS on the zonal localization of Mrp2 and Mrp3 and the expression of Mrp3, Mrp4, Mrp5, and Mrp6 mRNA were investigated in rat liver. In normal rat liver Mrp3 was found in pericentral hepatocytes also expressing glutamine synthetase. In LPS-treated rat liver the decrease in Mrp2 protein was most pronounced in pericentral hepatocytes, with only minor down-regulation in periportal hepatocytes. Conversely, induction of Mrp3 was found in pericentral hepatocytes with a low expression of Mrp2. Furthermore, we found a strong induction of Mrp5 mRNA. Likewise, Mrp6 mRNA was upregulated, however Mrp6 protein expression was not significantly altered. It is concluded that Mrp3 is inversely regulated to Mrp2 in a zonal pattern and may compensate for the LPSinduced loss of Mrp2 in the perivenous area. Induction of pericentral Mrp3 and upregulation of Mrp5 mRNA may play an important role in the hepatocellular clearance of cholephilic substances and cyclic nucleotides accumulating after LPS treatment. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Donner M. G. aut Warskulat U. aut Saha N. aut Häussinger D. aut Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 331-339 1431-6730 385:3-4<331 2004 385 bchm https://doi.org/10.1515/BC.2004.029 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.029 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donner M. G. aut NATIONALLICENCE 700 1- Warskulat U. aut NATIONALLICENCE 700 1- Saha N. aut NATIONALLICENCE 700 1- Häussinger D. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 331-339 1431-6730 385:3-4<331 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter