caa a22 4500 37892012X CHVBK 20180305123603.0 cr unu---uuuuu 161128e20040413xx s 000 0 eng 10.1515/BC.2004.022 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.022 Expression of functional purinergic receptors in pulmonary neuroepithelial bodies and their role in hypoxia chemotransmission [Elektronische Daten] [X. W. Fu, C. A. Nurse, E. Cutz] Adenine nucleotides act through specific cell surface receptors to invoke a variety of biological responses. Here we show that cells of neuroepithelial bodies (NEB), presumed O2 airway sensors in neonatal hamster lung, express functional P2X receptors (P2XR). Positive immunostaining was detected in NEB cells using doublelabel immunohistochemistry with antibodies against P2X2 and P2X3 receptor subunits, which colocalized with serotonin (5-HT), a marker of NEB cells. For electrophysiological characterization of P2X2-R in NEB cells, fresh neonatal hamster lung slice preparation was used. Under wholecell patch clamp, perfusion with ATP induced a concentrationdependent, nondesensitizing inward current (EC50=12 uM). Perfusion with α, β-methylene ATP also induced a slowdesensitizing inward current (EC50=8.2 uM). Suramin (IC50 ca. 43 M) and TNPATP (IC50 ca. 8 uM) blocked the currents evoked by both ATP and α, βmethylene ATP. Using carbon fiber amperometry we observed that hypoxia and ATP induced 5-HT release from NEB cells and that this release was blocked by suramin. These data suggest that functional P2X2/3 heteromeric receptors are expressed in NEB cells. The possible function of these purinoreceptors in NEB cells could include modulation of hypoxia chemotransmission. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Fu X. W. aut Nurse C. A. aut Cutz E. aut Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 275-284 1431-6730 385:3-4<275 2004 385 bchm https://doi.org/10.1515/BC.2004.022 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.022 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fu X. W. aut NATIONALLICENCE 700 1- Nurse C. A. aut NATIONALLICENCE 700 1- Cutz E. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/3-4(2004-04-13), 275-284 1431-6730 385:3-4<275 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter