caa a22 4500 378921657 CHVBK 20180305123606.0 cr unu---uuuuu 161128e20040101xx s 000 0 eng 10.1351/pac200476071571 doi (NATIONALLICENCE)gruyter-10.1351/pac200476071571 Selection and characterization of DNAzymes with synthetically appended functionalities: A case of a synthetic RNAsea mimic [Elektronische Daten] [R. Ting, Leonard Lermer, J. Thomas, Y. Roupioz, D. M. Perrin] We have been interested in merging synthetic nucleotide chemistry with combinatorial selection of DNAzymes to deliver a more complete (and complex) chemical complement to the catalytic repertoire of nucleic acids. Thus we ask, what do modified dNTPs really bring to nucleic acids in terms of an increased repertoire? In asking this question, we have looked first at conditions, and more recently for reaction classes where nucleic acids are found to be catalytically inefficient, deficient, or at least to date, seemingly incapable of certain functions. A case of this is M2+-independent ribophosphodiester hydrolysis at physiological pH and low ionic strength where nucleic acids exhibit especially low rate constants for self-cleavage and seem to be incapable of turnover. © 2013 Walter de Gruyter GmbH, Berlin/Boston Ting R. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut Lermer Leonard Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut Thomas J. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut Roupioz Y. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut Perrin D. M. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut Pure and Applied Chemistry De Gruyter 76/7-8(2004-01-01), 1571-1577 0033-4545 76:7-8<1571 2004 76 pac https://doi.org/10.1351/pac200476071571 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200476071571 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ting R. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut NATIONALLICENCE 700 1- Lermer Leonard Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut NATIONALLICENCE 700 1- Thomas J. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut NATIONALLICENCE 700 1- Roupioz Y. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut NATIONALLICENCE 700 1- Perrin D. M. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada aut NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 76/7-8(2004-01-01), 1571-1577 0033-4545 76:7-8<1571 2004 76 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter