Engineering DNA aptamers and DNA enzymes with fluorescence-signaling properties
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| 024 | 7 | 0 | |a 10.1351/pac200476071547 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1351/pac200476071547 | ||
| 245 | 0 | 0 | |a Engineering DNA aptamers and DNA enzymes with fluorescence-signaling properties |h [Elektronische Daten] |c [R. Nutiu, Shirley Mei, Zhongjie Liu, Y. Li] |
| 520 | 3 | |a Single-stranded DNA molecules with ligand-binding ability and catalytic function, referred to as DNA aptamers and DNA enzymes, respectively, are special DNA sequences isolated from random-sequence DNA libraries by "in vitro selection”. These two new classes of artificial DNA molecules have the potential of being used as molecular tools in a variety of innovative applications ranging from biosensing to gene regulation. Our laboratory is interested in engineering fluorescence-signaling DNA aptamers and DNA enzymes that can be widely exploited for detection-directed applications. In this article, we will first discuss our recent efforts on the rational design of a new class of signaling aptamers denoted "structure- switching signaling aptamers”, which report target binding by switching structures from DNA/DNA duplex to DNA/target complex. We will then describe the in vitro selection of fluorescence-signaling DNA enzymes that exhibit a synchronized catalysis-signaling capability by cleaving a chimeric RNA/DNA substrate at the lone RNA linkage surrounded by closely spaced fluorophore-quencher pair. Potential utilities of these signaling DNA molecules will also be discussed. | |
| 540 | |a © 2013 Walter de Gruyter GmbH, Berlin/Boston | ||
| 700 | 1 | |a Nutiu |D R. |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | |
| 700 | 1 | |a Mei |D Shirley |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | |
| 700 | 1 | |a Liu |D Zhongjie |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | |
| 700 | 1 | |a Li |D Y. |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | |
| 773 | 0 | |t Pure and Applied Chemistry |d De Gruyter |g 76/7-8(2004-01-01), 1547-1561 |x 0033-4545 |q 76:7-8<1547 |1 2004 |2 76 |o pac | |
| 856 | 4 | 0 | |u https://doi.org/10.1351/pac200476071547 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1351/pac200476071547 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nutiu |D R. |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mei |D Shirley |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Zhongjie |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Y. |u Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Pure and Applied Chemistry |d De Gruyter |g 76/7-8(2004-01-01), 1547-1561 |x 0033-4545 |q 76:7-8<1547 |1 2004 |2 76 |o pac | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||