caa a22 4500 378921703 CHVBK 20180305123607.0 cr unu---uuuuu 161128e20040101xx s 000 0 eng 10.1351/pac200476071547 doi (NATIONALLICENCE)gruyter-10.1351/pac200476071547 Engineering DNA aptamers and DNA enzymes with fluorescence-signaling properties [Elektronische Daten] [R. Nutiu, Shirley Mei, Zhongjie Liu, Y. Li] Single-stranded DNA molecules with ligand-binding ability and catalytic function, referred to as DNA aptamers and DNA enzymes, respectively, are special DNA sequences isolated from random-sequence DNA libraries by "in vitro selection”. These two new classes of artificial DNA molecules have the potential of being used as molecular tools in a variety of innovative applications ranging from biosensing to gene regulation. Our laboratory is interested in engineering fluorescence-signaling DNA aptamers and DNA enzymes that can be widely exploited for detection-directed applications. In this article, we will first discuss our recent efforts on the rational design of a new class of signaling aptamers denoted "structure- switching signaling aptamers”, which report target binding by switching structures from DNA/DNA duplex to DNA/target complex. We will then describe the in vitro selection of fluorescence-signaling DNA enzymes that exhibit a synchronized catalysis-signaling capability by cleaving a chimeric RNA/DNA substrate at the lone RNA linkage surrounded by closely spaced fluorophore-quencher pair. Potential utilities of these signaling DNA molecules will also be discussed. © 2013 Walter de Gruyter GmbH, Berlin/Boston Nutiu R. Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut Mei Shirley Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut Liu Zhongjie Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut Li Y. Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut Pure and Applied Chemistry De Gruyter 76/7-8(2004-01-01), 1547-1561 0033-4545 76:7-8<1547 2004 76 pac https://doi.org/10.1351/pac200476071547 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200476071547 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nutiu R. Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut NATIONALLICENCE 700 1- Mei Shirley Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut NATIONALLICENCE 700 1- Liu Zhongjie Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut NATIONALLICENCE 700 1- Li Y. Department of Biochemistry and Department of Chemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada aut NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 76/7-8(2004-01-01), 1547-1561 0033-4545 76:7-8<1547 2004 76 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter