caa a22 4500 378927299 CHVBK 20180305123620.0 cr unu---uuuuu 161128e20041101xx s 000 0 eng 10.1524/ract.92.9.741.55006 doi (NATIONALLICENCE)gruyter-10.1524/ract.92.9.741.55006 Sorption behavior of europium(III) and curium(III) on the cell surfaces of microorganisms [Elektronische Daten] [Takuo Ozaki, J. B. Gillow, Takaumi Kimura, T. Ohnuki, Zenko Yoshida, Arokiasamy J. Francis] We investigated the association of europium(III) and curium(III) with the microorganisms Chlorella vulgaris, Bacillus subtilis, Pseudomonas fluorescens, Halomonas sp., Halobacterium salinarum, and Halobacterium halobium . We determined the kinetics and distribution coefficients (Kd) for Eu(III) and Cm(III) sorption at pH 3-5 by batch experiments, and evaluated the number of water molecules in the inner-sphere (NH₂O) and the degree of strength of ligand field (RE/M) for Eu(III) by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Exudates from C. vulgaris, Halomonas sp., and H. halobium had an affinity for Eu(III) and Cm(III). The log Kd of Eu(III) and Cm(III) showed that their sorption was not fully due to the exchange with three protons on the functional groups on cell surfaces. The halophilic microorganisms (Halomonas sp., Halobacterium salinarum, H. halobium) showed almost no pH dependence in log Kd, indicating that an exchange with Na+ on the functional groups was involved in their sorption. The Δ NH₂O(=9-NH₂O) for Eu(III) on C. vulgaris was 1-3, while that for the other microorganisms was over 3, demonstrating that the coordination of Eu(III) with C. vulgaris was predominantly an outer-spherical process. The RE/M for Eu(III) on halophilic microorganisms was 2.5-5, while that for non-halophilic ones was 1-2.5. This finding suggests that the coordination environment of Eu(III) on the halophilic microorganisms is more complicated than that on the other three non-halophilic ones. © 2004 Oldenbourg Wissenschaftsverlag GmbH Laboratory techniques, experiments nationallicence Analytical chemistry nationallicence Inorganic chemistry nationallicence Ozaki Takuo aut Gillow J. B. aut Kimura Takaumi aut Ohnuki T. aut Yoshida Zenko aut Francis Arokiasamy J. aut Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 92/9-11/2004(2004-11-01), 741-748 0033-8230 92:9-11/2004<741 2004 92 ract https://doi.org/10.1524/ract.92.9.741.55006 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1524/ract.92.9.741.55006 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ozaki Takuo aut NATIONALLICENCE 700 1- Gillow J. B. aut NATIONALLICENCE 700 1- Kimura Takaumi aut NATIONALLICENCE 700 1- Ohnuki T. aut NATIONALLICENCE 700 1- Yoshida Zenko aut NATIONALLICENCE 700 1- Francis Arokiasamy J. aut NATIONALLICENCE 773 0- Radiochimica Acta/International journal for chemical aspects of nuclear science and technology Oldenbourg Wissenschaftsverlag GmbH 92/9-11/2004(2004-11-01), 741-748 0033-8230 92:9-11/2004<741 2004 92 ract CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter