caa a22 4500 378931644 CHVBK 20180305123631.0 cr unu---uuuuu 161128e20040101xx s 000 0 eng 10.1351/pac200476040879 doi (NATIONALLICENCE)gruyter-10.1351/pac200476040879 In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling [Elektronische Daten] [N. Torto, M. Ohlrogge, L. Gorton, J. M. Van Alstine, Thomas Laurell, G. Marko-Varga] A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyme complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications. © 2013 Walter de Gruyter GmbH, Berlin/Boston Torto N. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut Ohlrogge M. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut Gorton L. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut Van Alstine J. M. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut Laurell Thomas Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut Marko-Varga G. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut Pure and Applied Chemistry De Gruyter 76/4(2004-01-01), 879-888 0033-4545 76:4<879 2004 76 pac https://doi.org/10.1351/pac200476040879 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200476040879 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Torto N. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Ohlrogge M. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Gorton L. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Van Alstine J. M. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Laurell Thomas Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Marko-Varga G. Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone, Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden aut NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 76/4(2004-01-01), 879-888 0033-4545 76:4<879 2004 76 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter