caa a22 4500 378931725 CHVBK 20180305123631.0 cr unu---uuuuu 161128e20040101xx s 000 0 eng 10.1351/pac200476040765 doi (NATIONALLICENCE)gruyter-10.1351/pac200476040765 Glutamate oxidase advances in the selective bioanalytical detection of the neurotoxic amino acid β-ODAP in grass pea: A decade of progress [Elektronische Daten] [G. Moges, N. Wodajo, L. Gorton, Yirgalem Yigzaw, Kurt Kalcher, Abebaw Belay, Girma Akalu, B. M. Nair, T. Solomon] The search for an enzyme as a reagent for selective bioanalytical detection of the neurotoxic amino acid, β-N-oxalyl L-alpha, β-diaminopropionic acid, β-ODAP (found in grass pea, Lathyrus sativus) led to its redox catalytic reaction by glutamate oxidase (GluOx). Homogeneous kinetic studies and an immobilized GluOx reactor-based flow-injection assay were initially made for beta-ODAP with small immobilized GluOx/catalase glutamate destroying prereactors. The method was applied to examine the toxin content in processed grass pea. The kinetics and the equilibrium of the thermal isomerization of β-ODAP to the nontoxic isomer α-ODAP established that GluOx is specific to the neurotoxin. The first ever GluOx-based amperometric biosensor for liquid chromatography (LC) detection was reported in 1997. This biosensor coupled with a refractive index detector improved LC performance. The most recent work with GluOx resulted in MnO2-based screen-printed amperometric biosensor, with offline elimination of glutamate interference by glutamate decarboxylase. A single-shot chemiluminescent sensor developed for hydrogen peroxide is also proposed for β-ODAP with GluOx application. This decade of progress resulted from studies that included four Ph.D. (Ethiopia, Sweden, Austria), four M.Sc. (Ethiopia, Sweden) and Licentiate (Sweden) theses projects, plus one collaborative project in Sweden. The advances in grass pea research may be regarded as a model north-south cooperation for research and education. © 2013 Walter de Gruyter GmbH, Berlin/Boston Moges G. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Wodajo N. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Gorton L. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Yigzaw Yirgalem Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Kalcher Kurt Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Belay Abebaw Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Akalu Girma Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Nair B. M. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Solomon T. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut Pure and Applied Chemistry De Gruyter 76/4(2004-01-01), 765-775 0033-4545 76:4<765 2004 76 pac https://doi.org/10.1351/pac200476040765 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200476040765 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moges G. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Wodajo N. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Gorton L. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Yigzaw Yirgalem Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Kalcher Kurt Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Belay Abebaw Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Akalu Girma Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Nair B. M. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 700 1- Solomon T. Department of Chemistry, Addis Ababa, Ethiopia; Department of Analytical Chemistry, Institute of Chemistry, Karl-Franzens University, Graz, Austria; Department of Analytical Chemistry, University of Lund, Sweden; Applied Nutrition and Food Chemistry, University of Lund, Sweden aut NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 76/4(2004-01-01), 765-775 0033-4545 76:4<765 2004 76 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter