caa a22 4500 378931865 CHVBK 20180305123631.0 cr unu---uuuuu 161128e20040701xx s 000 0 eng 10.1515/BOT.2004.032 doi (NATIONALLICENCE)gruyter-10.1515/BOT.2004.032 Polyphosphate and siliceous granules in the macroscopic gametophytes of the red alga Porphyra purpurea (Bangiophyceae, Rhodophyta) [Elektronische Daten] [Thierry Chopin, Tania Morais, Ellen Belyea, Susan Belfry] Phosphorylated fractions in tissues of starved and fresh plants of Porphyra purpurea, a representative of the Bangiophyceae, were analyzed over a 48 h incubation period in pulse-enriched seawater (15 uM phosphorus and 25 uM nitrogen). Compared to Chondrus crispus, a representative of the structurally and reproductively more complex Florideophyceae, P. purpurea takes up phosphorus much more actively, with a higher turnover rate, in which the orthophosphate fraction is predominant and acid-soluble and acid-insoluble polyphosphates are less significant as storage pools. Presence of cytoplasmic acid-insoluble polyphosphate granules was confirmed by transmission electron microscopy and energy dispersive X-ray microanalysis. The granules in P. purpurea were much smaller (20-110 nm in diameter) than those in C. crispus (around 1 um, but some larger than 2 um). Larger granules (290-310 nm) were observed in P. purpurea. They were more uniformly electron-opaque, without the reticulated/globular appearance of typical polyphosphate granules. Energy dispersive X-ray microanalysis demonstrated they were siliceous granules. To our knowledge, this is the first report in algae of such structures whose occurrence and metabolic role remain enigmatic. © Walter de Gruyter Hydrobiology nationallicence Mycology, fungi nationallicence Botany & plant sciences nationallicence Chondrus crispus nationallicence polyphosphates nationallicence Porphyra purpurea nationallicence Rhodophyta nationallicence siliceous granules nationallicence Chopin Thierry University of New Brunswick, Centre for Coastal Studies and Aquaculture and Centre for Environmental and Molecular Algal Research, P.O. Box 5050, Saint John, N.B., E2L 4L5, Canada aut Morais Tania University of New Brunswick, Centre for Coastal Studies and Aquaculture and Centre for Environmental and Molecular Algal Research, P.O. Box 5050, Saint John, N.B., E2L 4L5, Canada aut Belyea Ellen University of New Brunswick, Centre for Coastal Studies and Aquaculture and Centre for Environmental and Molecular Algal Research, P.O. Box 5050, Saint John, N.B., E2L 4L5, Canada aut Belfry Susan University of New Brunswick, Electron MicroscopyUnit, P.O. Box 45111, Fredericton, N.B., E3B 6E1,Canada aut Botanica Marina Walter de Gruyter 47/4(2004-07-01), 272-280 0006-8055 47:4<272 2004 47 botm https://doi.org/10.1515/BOT.2004.032 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BOT.2004.032 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chopin Thierry University of New Brunswick, Centre for Coastal Studies and Aquaculture and Centre for Environmental and Molecular Algal Research, P.O. Box 5050, Saint John, N.B., E2L 4L5, Canada aut NATIONALLICENCE 700 1- Morais Tania University of New Brunswick, Centre for Coastal Studies and Aquaculture and Centre for Environmental and Molecular Algal Research, P.O. Box 5050, Saint John, N.B., E2L 4L5, Canada aut NATIONALLICENCE 700 1- Belyea Ellen University of New Brunswick, Centre for Coastal Studies and Aquaculture and Centre for Environmental and Molecular Algal Research, P.O. Box 5050, Saint John, N.B., E2L 4L5, Canada aut NATIONALLICENCE 700 1- Belfry Susan University of New Brunswick, Electron MicroscopyUnit, P.O. Box 45111, Fredericton, N.B., E3B 6E1,Canada aut NATIONALLICENCE 773 0- Botanica Marina Walter de Gruyter 47/4(2004-07-01), 272-280 0006-8055 47:4<272 2004 47 botm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter