caa a22 4500 378938142 CHVBK 20180305123645.0 cr unu---uuuuu 161128e20040709xx s 000 0 eng 10.1515/JPM.2004.066 doi (NATIONALLICENCE)gruyter-10.1515/JPM.2004.066 Evidence of fetal pulmonary aspiration of intra-amniotic administered surfactant in animal experiment [Elektronische Daten] [R. Illia, C. Solana, P. Oliveri, J. Toblli, M.U. Imaz, D. Häbich] Introduction: The instillation of surfactant into the airways of patients with respiratory distress syndrome (RDS), especially in the neonatal period, is a proven therapy. The preventive therapy of RDS through intra-amniotic injection of surfactant has been reported recently. It has not been conclusively shown, however, that the surfactant administered in this way actually reaches the fetal pulmonary airways. Objective: To study the distribution in fetal organs of a natural surfactant labeled with technetium-99m and injected through amniocentesis into the amniotic sac of guinea pigs in the last third of pregnancy. Methods: After stimulating fetal respiratory movements with aminophylline 0.3 ml of an aqueous suspension containing 0.75 mg of phospholipids of a natural bovine surfactant labeled with technetium-99m, together with 0.1 ml of the biological dye carmine indigo, were injected into the amniotic sac. One hour later fetuses were delivered by cesarean section. In those that were dye-stained, dosimetric and gammagraphic tests were applied to trachea, lungs, esophagus, stomach, heart, liver, kidneys and placenta. Results: Significant radio isotopic activity was found in both lungs of six treated fetuses, with a dose capture of between 1.0% and 5.3% of total dose. The level of activity in the stomachs was similar to that in the lungs (0.9% to 3.0% dose capture), whereas activity in other organs was negligible except in two placentae. No radio isotopic activity was found in non-injected control fetuses. Conclusions: In the present animal model natural surfactant injected intra-amniotically is aspirated into the lungs within one hour. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Human reproduction, growth & development nationallicence Gynaecology & obstetrics nationallicence Paediatric medicine nationallicence Illia R. aut Solana C. aut Oliveri P. aut Toblli J. aut Imaz M.U. aut Häbich D. aut Journal of Perinatal Medicine Walter de Gruyter 32/4(2004-07-09), 354-358 0300-5577 32:4<354 2004 32 jpme https://doi.org/10.1515/JPM.2004.066 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/JPM.2004.066 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Illia R. aut NATIONALLICENCE 700 1- Solana C. aut NATIONALLICENCE 700 1- Oliveri P. aut NATIONALLICENCE 700 1- Toblli J. aut NATIONALLICENCE 700 1- Imaz M.U. aut NATIONALLICENCE 700 1- Häbich D. aut NATIONALLICENCE 773 0- Journal of Perinatal Medicine Walter de Gruyter 32/4(2004-07-09), 354-358 0300-5577 32:4<354 2004 32 jpme CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter