caa a22 4500 378940961 CHVBK 20180305123652.0 cr unu---uuuuu 161128e20041001xx s 000 0 eng 10.1515/BC.2004.125 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.125 A database search for double-strand containing RNAs in Dictyostelium discoideum [Elektronische Daten] [Stefan Gräf, Branimira E. Borisova, Wolfgang Nellen, Gerhard Steger, Christian Hammann] In eukaryotic cells, double-stranded RNA is degraded to 21mers and triggers RNA interference. Using a pattern description language, we have searched the EMBL database for sequences with the potential to form double strands in cis in Dictyostelium discoideum. No extended inverted repeats were found in mRNAs. However, the antisense direction of some mRNAs encoding regulatory or developmentally regulated proteins showed the ability to form double-stranded regions. In EST archives, we found potential double strands derived from a few genes, but these transcripts are not continuously encoded in the genome. Most likely, they represent hybrid molecules of sense and antisense RNAs. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence gene regulation nationallicence genome analysis nationallicence pattern search nationallicence RNAi nationallicence siRNAs nationallicence Gräf Stefan Institut für Physikalische Biologie, Heinrich-Heine- Universität, D-40225 Düsseldorf, Germany aut Borisova Branimira E. Department of Genetics, AG Molecular Interactions, University of Kassel, D-34132 Kassel, Germany aut Nellen Wolfgang Department of Genetics, University of Kassel, D-34132 Kassel, Germany aut Steger Gerhard Institut für Physikalische Biologie, Heinrich-Heine- Universität, D-40225 Düsseldorf, Germany aut Hammann Christian Department of Genetics, AG Molecular Interactions, University of Kassel, D-34132 Kassel, Germany aut Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 961-965 1431-6730 385:10<961 2004 385 bchm https://doi.org/10.1515/BC.2004.125 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.125 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gräf Stefan Institut für Physikalische Biologie, Heinrich-Heine- Universität, D-40225 Düsseldorf, Germany aut NATIONALLICENCE 700 1- Borisova Branimira E. Department of Genetics, AG Molecular Interactions, University of Kassel, D-34132 Kassel, Germany aut NATIONALLICENCE 700 1- Nellen Wolfgang Department of Genetics, University of Kassel, D-34132 Kassel, Germany aut NATIONALLICENCE 700 1- Steger Gerhard Institut für Physikalische Biologie, Heinrich-Heine- Universität, D-40225 Düsseldorf, Germany aut NATIONALLICENCE 700 1- Hammann Christian Department of Genetics, AG Molecular Interactions, University of Kassel, D-34132 Kassel, Germany aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 961-965 1431-6730 385:10<961 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter