caa a22 4500 37894097X CHVBK 20180305123652.0 cr unu---uuuuu 161128e20041001xx s 000 0 eng 10.1515/BC.2004.119 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.119 Steinhoff Heinz-Jürgen 1. Fachbereich Physik, Universität Osnabrück, D-49069 Osnabrück, Germany Inter- and intra-molecular distances determined by EPR spectroscopy and site-directed spin labeling reveal protein-protein and protein-oligonucleotide interaction [Elektronische Daten] [Heinz-Jürgen Steinhoff] Recent developments including pulse and multi-frequency techniques make the combination of site-directed spin labeling and electron paramagnetic resonance (EPR) spectroscopy an attractive approach for the study of protein-protein or protein-oligonucleotide interaction. Analysis of the spin label side chain mobility, its solvent accessibility, the polarity of the spin label micro-environment and distances between spin label side chains allow the modeling of protein domains or protein-protein interaction sites and their conformational changes with a spatial resolution at the level of the backbone fold. Structural changes can be detected with millisecond time resolution. Inter- and intra-molecular distances are accessible in the range from approximately 0.5 to 8 nm by the combination of continuous wave and pulse EPR methods. Recent applications include the study of transmembrane substrate transport, membrane channel gating, gene regulation and signal transfer. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence bacteriorhodopsin nationallicence inter-spin distance nationallicence proline permease nationallicence pulse EPR nationallicence sensory rhodopsin nationallicence Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 913-920 1431-6730 385:10<913 2004 385 bchm https://doi.org/10.1515/BC.2004.119 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.119 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Steinhoff Heinz-Jürgen 1. Fachbereich Physik, Universität Osnabrück, D-49069 Osnabrück, Germany NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 913-920 1431-6730 385:10<913 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter