Generation of glycogen- and albumin-producing hepatocyte-like cells from embryonic stem cells
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 378941070 | ||
| 003 | CHVBK | ||
| 005 | 20180305123652.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 161128e20041001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1515/BC.2004.123 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.123 | ||
| 245 | 0 | 0 | |a Generation of glycogen- and albumin-producing hepatocyte-like cells from embryonic stem cells |h [Elektronische Daten] |c [Gabriela Kania, Przemyslaw Blyszczuk, Andrea Jochheim, Michael Ott, Anna M. Wobus] |
| 520 | 3 | |a We present a novel two-step protocol for the differentiation of embryonic stem (ES) cells into the hepatic lineage. Differentiated hepatocyte-like cells express genes and proteins characteristic for endodermal and hepatic cells and acquire a functional hepatic phenotype as demonstrated by albumin secretion and glycogen storage. During differentiation, α-fetoprotein, albumin, transthyretin, α-1-antitrypsin, cytochrome P450 subunits 2b9 and 2b13 and tyrosine aminotransferase transcripts are upregulated. Quantitative RT-PCR data revealed a fetal hepatic phenotype corresponding to day 13-14 of liver development. Terminally differentiated hepatocyte-like cells show a bi-nucleated, cuboidal morphology labeled by albumin, α-1-antitrypsin, liver amylase, dipeptidyl peptidase IV, c-met and cytokeratin 18. ES-derived intermediate cell types transiently and partially co-express nestin with albumin and α-fetoprotein, respectively, but not cytokeratin 19. This finding suggests an ES-derived potential hepatic progenitor cell type, which is partially nestin-, albumin- and α-fetoproteinpositive, but cytokeratin 19-negative. | |
| 540 | |a © Walter de Gruyter | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 690 | 7 | |a albumin |2 nationallicence | |
| 690 | 7 | |a α-fetoprotein |2 nationallicence | |
| 690 | 7 | |a embryonic stem cells |2 nationallicence | |
| 690 | 7 | |a hepatic differentiation |2 nationallicence | |
| 690 | 7 | |a mouse |2 nationallicence | |
| 690 | 7 | |a nestin |2 nationallicence | |
| 700 | 1 | |a Kania |D Gabriela |u In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany |4 aut | |
| 700 | 1 | |a Blyszczuk |D Przemyslaw |u In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany |4 aut | |
| 700 | 1 | |a Jochheim |D Andrea |u Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany |4 aut | |
| 700 | 1 | |a Ott |D Michael |u Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany |4 aut | |
| 700 | 1 | |a Wobus |D Anna M. |u In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/10(2004-10-01), 943-953 |x 1431-6730 |q 385:10<943 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.123 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.123 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kania |D Gabriela |u In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Blyszczuk |D Przemyslaw |u In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jochheim |D Andrea |u Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ott |D Michael |u Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wobus |D Anna M. |u In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/10(2004-10-01), 943-953 |x 1431-6730 |q 385:10<943 |1 2004 |2 385 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||