caa a22 4500 378941070 CHVBK 20180305123652.0 cr unu---uuuuu 161128e20041001xx s 000 0 eng 10.1515/BC.2004.123 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.123 Generation of glycogen- and albumin-producing hepatocyte-like cells from embryonic stem cells [Elektronische Daten] [Gabriela Kania, Przemyslaw Blyszczuk, Andrea Jochheim, Michael Ott, Anna M. Wobus] We present a novel two-step protocol for the differentiation of embryonic stem (ES) cells into the hepatic lineage. Differentiated hepatocyte-like cells express genes and proteins characteristic for endodermal and hepatic cells and acquire a functional hepatic phenotype as demonstrated by albumin secretion and glycogen storage. During differentiation, α-fetoprotein, albumin, transthyretin, α-1-antitrypsin, cytochrome P450 subunits 2b9 and 2b13 and tyrosine aminotransferase transcripts are upregulated. Quantitative RT-PCR data revealed a fetal hepatic phenotype corresponding to day 13-14 of liver development. Terminally differentiated hepatocyte-like cells show a bi-nucleated, cuboidal morphology labeled by albumin, α-1-antitrypsin, liver amylase, dipeptidyl peptidase IV, c-met and cytokeratin 18. ES-derived intermediate cell types transiently and partially co-express nestin with albumin and α-fetoprotein, respectively, but not cytokeratin 19. This finding suggests an ES-derived potential hepatic progenitor cell type, which is partially nestin-, albumin- and α-fetoproteinpositive, but cytokeratin 19-negative. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence albumin nationallicence α-fetoprotein nationallicence embryonic stem cells nationallicence hepatic differentiation nationallicence mouse nationallicence nestin nationallicence Kania Gabriela In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany aut Blyszczuk Przemyslaw In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany aut Jochheim Andrea Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany aut Ott Michael Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany aut Wobus Anna M. In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany aut Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 943-953 1431-6730 385:10<943 2004 385 bchm https://doi.org/10.1515/BC.2004.123 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.123 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kania Gabriela In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany aut NATIONALLICENCE 700 1- Blyszczuk Przemyslaw In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany aut NATIONALLICENCE 700 1- Jochheim Andrea Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany aut NATIONALLICENCE 700 1- Ott Michael Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany aut NATIONALLICENCE 700 1- Wobus Anna M. In vitro Differentiation Group, IPK Gatersleben, D-06466 Gatersleben, Germany aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 943-953 1431-6730 385:10<943 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter