caa a22 4500 378941089 CHVBK 20180305123652.0 cr unu---uuuuu 161128e20041001xx s 000 0 eng 10.1515/BC.2004.124 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.124 Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM [Elektronische Daten] [A.S.M. Kamruzzahan, Ferry Kienberger, Cordula M. Stroh, Jörg Berg, Ralf Huss, Andreas Ebner, Rong Zhu, Christian Rankl, Hermann J. Gruber, Peter Hinterdorfer] The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. This results in a tight but non-invasive attachment of the cells. Using tapping-mode AFM, which is known as gentle imaging mode and therefore most appropriate for soft biological samples like erythrocytes, it was possible to resolve membrane skeleton structures without major distortions or deformations of the cell surface. Significant differences in the morphology of RBCs from healthy humans and patients with systemic lupus erythematosus (SLE) were observed on topographical images. The surface of RBCs from SLE patients showed characteristic circular-shaped holes with approx. 200 nm in diameter under physiological conditions, a possible morphological correlate to previously published changes in the SLE erythrocyte membrane. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence AFM nationallicence erythrocytes nationallicence imaging nationallicence molecular recognition nationallicence red blood cells nationallicence SLE nationallicence Kamruzzahan A.S.M. Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Kienberger Ferry Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Stroh Cordula M. Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Berg Jörg Institute for Medical Diagnostics and Immunologie, General Hospital of Linz, Krankenhaus Strasse 9, A-4020 Linz, Austria aut Huss Ralf Institute of Pathology, University of Munich, Thalkirchner Str. 36, D-80337 Munich, Germany aut Ebner Andreas Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Zhu Rong Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Rankl Christian Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Gruber Hermann J. Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Hinterdorfer Peter Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 955-960 1431-6730 385:10<955 2004 385 bchm https://doi.org/10.1515/BC.2004.124 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.124 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamruzzahan A.S.M. Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Kienberger Ferry Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Stroh Cordula M. Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Berg Jörg Institute for Medical Diagnostics and Immunologie, General Hospital of Linz, Krankenhaus Strasse 9, A-4020 Linz, Austria aut NATIONALLICENCE 700 1- Huss Ralf Institute of Pathology, University of Munich, Thalkirchner Str. 36, D-80337 Munich, Germany aut NATIONALLICENCE 700 1- Ebner Andreas Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Zhu Rong Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Rankl Christian Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Gruber Hermann J. Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 700 1- Hinterdorfer Peter Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 955-960 1431-6730 385:10<955 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter