Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis
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| 024 | 7 | 0 | |a 10.1515/BC.2004.126 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2004.126 | ||
| 245 | 0 | 0 | |a Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis |h [Elektronische Daten] |c [Masahiko Shiraishi, Adam J. Oates, Xu Li, Ying H. Chuu, Takao Sekiya] |
| 520 | 3 | |a The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed. | |
| 540 | |a © Walter de Gruyter | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 690 | 7 | |a denaturing gradient gel electrophoresis |2 nationallicence | |
| 690 | 7 | |a DNA methylation |2 nationallicence | |
| 690 | 7 | |a gene identification |2 nationallicence | |
| 690 | 7 | |a relative helix survival time |2 nationallicence | |
| 690 | 7 | |a strand dissociation kinetics |2 nationallicence | |
| 700 | 1 | |a Shiraishi |D Masahiko |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | |
| 700 | 1 | |a Oates |D Adam J. |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | |
| 700 | 1 | |a Li |D Xu |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | |
| 700 | 1 | |a Chuu |D Ying H. |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | |
| 700 | 1 | |a Sekiya |D Takao |u Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 385/10(2004-10-01), 967-973 |x 1431-6730 |q 385:10<967 |1 2004 |2 385 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2004.126 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2004.126 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shiraishi |D Masahiko |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oates |D Adam J. |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Xu |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chuu |D Ying H. |u DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sekiya |D Takao |u Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 385/10(2004-10-01), 967-973 |x 1431-6730 |q 385:10<967 |1 2004 |2 385 |o bchm | ||
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