caa a22 4500 378941100 CHVBK 20180305123652.0 cr unu---uuuuu 161128e20041001xx s 000 0 eng 10.1515/BC.2004.126 doi (NATIONALLICENCE)gruyter-10.1515/BC.2004.126 Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis [Elektronische Daten] [Masahiko Shiraishi, Adam J. Oates, Xu Li, Ying H. Chuu, Takao Sekiya] The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed. © Walter de Gruyter Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence denaturing gradient gel electrophoresis nationallicence DNA methylation nationallicence gene identification nationallicence relative helix survival time nationallicence strand dissociation kinetics nationallicence Shiraishi Masahiko DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut Oates Adam J. DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut Li Xu DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut Chuu Ying H. DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut Sekiya Takao Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan aut Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 967-973 1431-6730 385:10<967 2004 385 bchm https://doi.org/10.1515/BC.2004.126 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2004.126 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shiraishi Masahiko DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut NATIONALLICENCE 700 1- Oates Adam J. DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut NATIONALLICENCE 700 1- Li Xu DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut NATIONALLICENCE 700 1- Chuu Ying H. DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan aut NATIONALLICENCE 700 1- Sekiya Takao Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 385/10(2004-10-01), 967-973 1431-6730 385:10<967 2004 385 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter