Organisation of the microtubular cytoskeleton in protoplasts from Palmaria palmata (Palmariales, Rhodophyta)

Verfasser / Beitragende:
[Line Le Gall, Anne-Marie Rusig, Joël Cosson]
Ort, Verlag, Jahr:
2004
Enthalten in:
Botanica Marina, 47/3(2004-07-12), 231-237
Format:
Artikel (online)
ID: 378941585
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245 0 0 |a Organisation of the microtubular cytoskeleton in protoplasts from Palmaria palmata (Palmariales, Rhodophyta)  |h [Elektronische Daten]  |c [Line Le Gall, Anne-Marie Rusig, Joël Cosson] 
520 3 |a Confocal laser scanning microscopy was used to investigate the three-dimensional organisation of the microtubular cytoskeleton of protoplasts isolated from the cortex and medulla of the red alga Palmaria palmata. Immunofluorescence labelling revealed the presence of abundant microtubules in interphase cells. Cortex-derived and medulla-derived protoplasts each exhibited a distinctive arrangement of microtubules. In cortex-derived protoplasts, a continuous network of cytoplasmic microtubules connected the peripheral array to the array which surrounded the single nucleus at the centre of the cell. At telophase, the microtubular cytoskeleton was reorganised into a network apparently involved in cytokinesis. In the multi-nucleate medulla-derived protoplasts, each nucleus, located near the plasma membrane, was surrounded by microtubules. A uniformly arranged cortical array of microtubules was observed in both cortex-derived and medulla-derived protoplasts during interphase and we hypothesise that it likely plays a role in the biogenesis of the cell wall in red algal cells. 
540 |a Copyright © 2004 by Walter de Gruyter GmbH & Co. KG 
690 7 |a Hydrobiology  |2 nationallicence 
690 7 |a Mycology, fungi  |2 nationallicence 
690 7 |a Botany & plant sciences  |2 nationallicence 
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