caa a22 4500 378941585 CHVBK 20180305123653.0 cr unu---uuuuu 161128e20040712xx s 000 0 eng 10.1515/BOT.2004.024 doi (NATIONALLICENCE)gruyter-10.1515/BOT.2004.024 Organisation of the microtubular cytoskeleton in protoplasts from Palmaria palmata (Palmariales, Rhodophyta) [Elektronische Daten] [Line Le Gall, Anne-Marie Rusig, Joël Cosson] Confocal laser scanning microscopy was used to investigate the three-dimensional organisation of the microtubular cytoskeleton of protoplasts isolated from the cortex and medulla of the red alga Palmaria palmata. Immunofluorescence labelling revealed the presence of abundant microtubules in interphase cells. Cortex-derived and medulla-derived protoplasts each exhibited a distinctive arrangement of microtubules. In cortex-derived protoplasts, a continuous network of cytoplasmic microtubules connected the peripheral array to the array which surrounded the single nucleus at the centre of the cell. At telophase, the microtubular cytoskeleton was reorganised into a network apparently involved in cytokinesis. In the multi-nucleate medulla-derived protoplasts, each nucleus, located near the plasma membrane, was surrounded by microtubules. A uniformly arranged cortical array of microtubules was observed in both cortex-derived and medulla-derived protoplasts during interphase and we hypothesise that it likely plays a role in the biogenesis of the cell wall in red algal cells. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Hydrobiology nationallicence Mycology, fungi nationallicence Botany & plant sciences nationallicence Gall Line Le aut Rusig Anne-Marie aut Cosson Joël aut Botanica Marina Walter de Gruyter 47/3(2004-07-12), 231-237 0006-8055 47:3<231 2004 47 botm https://doi.org/10.1515/BOT.2004.024 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BOT.2004.024 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gall Line Le aut NATIONALLICENCE 700 1- Rusig Anne-Marie aut NATIONALLICENCE 700 1- Cosson Joël aut NATIONALLICENCE 773 0- Botanica Marina Walter de Gruyter 47/3(2004-07-12), 231-237 0006-8055 47:3<231 2004 47 botm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter