caa a22 4500 378942409 CHVBK 20180305123655.0 cr unu---uuuuu 161128e20040301xx s 000 0 eng 10.1515/JPM.2004.025 doi (NATIONALLICENCE)gruyter-10.1515/JPM.2004.025 Is cervical dilatation during parturition at term associated with apoptosis? [Elektronische Daten] [B. Kemp, W. Rath, M. Winkler, T. Reineke, H.M. Beier, U. von Rango] Aims: Cellular turnover may be involved in remodeling of the cervix during parturition. Therefore, the number and localization of apoptotic and proliferating cells during cervical dilatation at term were determined. Methods: Biopsy specimens from the lower uterine segment of 36 women undergoing cesarean section with a cervical dilatation of <2 cm (n = 10), 2-<4 cm (n = 9), 4-6 cm (n = 8), and 6 cm (n = 9) were examined for nuclear fragmentation by the TUNEL assay, and for cell survival by the apoptosis-blocking bcl-2. Proliferation was marked by Ki-67, epithelial cells by cytokeratin and leukocytes by CD 45. For quantification of apoptotic and proliferating cells, eight random fields of each specimen stained for TUNEL or Ki-67 were blindly counted by two investigators. For statistical evaluation, 90% confidence intervals based on a Poisson distribution were used; groups with non-overlapping intervals were considered significantly different. Results: Apoptotic cells were found exclusively within the stromal compartment, while bcl-2 was expressed in epithelial cells and leukocytes. Proliferating cells were of stromal and epithelial origin. The number of apoptotic as well as proliferating cells ranged from 0 to 2 cells per high-power field (median number 0) in all groups. The confidence intervals were overlapping for all groups, showing no statistical difference between them. Conclusion: Apoptosis does not seem to play a decisive role in the process of cervical dilatation during parturition at term. © Walter de Gruyter Human reproduction, growth & development nationallicence Gynaecology & obstetrics nationallicence Paediatric medicine nationallicence Apoptosis nationallicence cervical dilatation nationallicence cervix nationallicence parturition nationallicence proliferation nationallicence Kemp B. Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany. aut Rath W. Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany. aut Winkler M. Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany. aut Reineke T. Statistical Institute, Aachen, Germany. aut Beier H.M. Institute of Anatomy and Reproductive Biology, University Hospital, Technical University, Aachen, Germany. aut Rango U. von Institute of Anatomy and Reproductive Biology, University Hospital, Technical University, Aachen, Germany. aut Journal of Perinatal Medicine Walter de Gruyter 32/2(2004-03-01), 137-143 0300-5577 32:2<137 2004 32 jpme https://doi.org/10.1515/JPM.2004.025 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/JPM.2004.025 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kemp B. Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany aut NATIONALLICENCE 700 1- Rath W. Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany aut NATIONALLICENCE 700 1- Winkler M. Department of Obstetrics and Gynecology, University Hospital, Technical University, Aachen, Germany aut NATIONALLICENCE 700 1- Reineke T. Statistical Institute, Aachen, Germany aut NATIONALLICENCE 700 1- Beier H.M. Institute of Anatomy and Reproductive Biology, University Hospital, Technical University, Aachen, Germany aut NATIONALLICENCE 700 1- Rango U. von Institute of Anatomy and Reproductive Biology, University Hospital, Technical University, Aachen, Germany aut NATIONALLICENCE 773 0- Journal of Perinatal Medicine Walter de Gruyter 32/2(2004-03-01), 137-143 0300-5577 32:2<137 2004 32 jpme CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 378942409 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter