caa a22 4500 378942417 CHVBK 20180305123655.0 cr unu---uuuuu 161128e20040315xx s 000 0 eng 10.1515/JPM.2004.023 doi (NATIONALLICENCE)gruyter-10.1515/JPM.2004.023 Oxytocic activity of thrombin: modulation of thrombin-induced gravid rat myometrial contractions by 5-hydroxytryptamine receptor antagonists [Elektronische Daten] [A. Adebiyi, P. G. Adaikan, R. N. V. Prasad] Background: Thrombin possesses potent oxytocic activity in vitro and in vivo. This activity has been proposed to play a role in post-parturitional uterine contractions and possibly, preterm birth related to intrauterine hemorrhage. Previous workers have demonstrated that cyclo-oxygenase pathway may not play a significant role in oxytocic activity of thrombin. However, the role of 5-hydroxytryptamine (a mediator of some of the biological activities of thrombin) in the oxytocic activity of thrombin is unknown. The present study therefore aimed to examine the possible involvement of 5-hydroxytryptamine in thrombin-induced myometrial contractions. Methods: Effect of 5-hydroxytryptamine receptor antagonists on thrombin-induced contractions of isolated gravid rat myometrium was studied using isolated tissue bath system. Results: Thrombin-induced myometrial contractions were significantly and concentration-dependently inhibited by ketanserin and methysergide. Furthermore, 12±2% increase in the force of contractions of gravid rat myometrium pre-contracted with 5-hydroxytryptamine (1 μM) was provoked by 1 U/ml of thrombin. Thrombin-induced augmentation of the uterine stimulating effect of 5-hydroxytryptamine was characterized by pronounced increase in the contractile tone. Conclusions: 5-hydroxytryptamine may possibly play a role in oxytocic activity of thrombin. Uterine hyperactivity associated with intrauterine hemorrhage could hence involve thrombin-induced 5-hydroxytryptamine production in the uterus. Copyright © 2004 by Walter de Gruyter GmbH & Co. KG Human reproduction, growth & development nationallicence Gynaecology & obstetrics nationallicence Paediatric medicine nationallicence Adebiyi A. aut Adaikan P. G. aut Prasad R. N. V. aut Journal of Perinatal Medicine Walter de Gruyter 32/2(2004-03-15), 126-131 0300-5577 32:2<126 2004 32 jpme https://doi.org/10.1515/JPM.2004.023 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/JPM.2004.023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adebiyi A. aut NATIONALLICENCE 700 1- Adaikan P. G. aut NATIONALLICENCE 700 1- Prasad R. N. V. aut NATIONALLICENCE 773 0- Journal of Perinatal Medicine Walter de Gruyter 32/2(2004-03-15), 126-131 0300-5577 32:2<126 2004 32 jpme CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter