caa a22 4500 386320454 CHVBK 20180307111624.0 cr unu---uuuuu 161130e198812 xx s 000 0 eng 10.1017/S0003356100003561 doi S0003356100003561 pii (NATIONALLICENCE)cambridge-10.1017/S0003356100003561 Rumen degradation of straw 6. Effect of polysaccharidase enzymes on degradation characteristics of ensiled rice straw [Elektronische Daten] The effects of three variables; polysaccharidase inclusion level (0, 5 and 10 g/kg), moisture content (500, 600 and 700 g/kg dry matter (DM), and particle size (20, 5 and 2 mm), on the quality and degradation characteristics of ensiled rice straw were investigated. The polysaccharidase product used was shown to contain a broad spectrum of activities against polysaccharides typical of plant material. The major activities present being: mixed link glucanase, xylanase and both endo- and exo-cellulase. The straw treated with polysaccharidase had a lower final pH (5·21, 4·87, 4·82; P < 0·01), higher concentrations of lactic acid (1·98, 3·90, 3·67 g/kg; P < 0·01), a lower content of neutral-detergent fibre (689, 630, 621 g/kg DM; P < 0·01) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P < 0·01) for the 0, 5 and 10 g/kg polysaccharidase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of three sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p = a + b (1 - e−ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was increased (P < 0·01) by the addition of polysaccharidase. The maximum potential degradability (a + b), however, was not altered by any of the treatments. The values for c were 0·0498, 0·0677 and 0·0817 per h and for (a + b) were 62·4, 62·1 and 62·8 g/100 g DM for the 0, 5 and 10 g/kg polysaccharidase enzyme additions respectively. It is concluded that the use of polysaccharidase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics. Copyright © British Society of Animal Science 1988 Nakashima Y. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB Ørskov E. R. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB Hotten P. M. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB Ambo K. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB Takase Y. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB Animal Production Cambridge University Press 47/3(1988-12), 421-427 0003-3561 47:3<421 1988 47 ASC https://doi.org/10.1017/S0003356100003561 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0003356100003561 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakashima Y. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB NATIONALLICENCE 700 1- Ørskov E. R. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB NATIONALLICENCE 700 1- Hotten P. M. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB NATIONALLICENCE 700 1- Ambo K. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB NATIONALLICENCE 700 1- Takase Y. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB NATIONALLICENCE 773 0- Animal Production Cambridge University Press 47/3(1988-12), 421-427 0003-3561 47:3<421 1988 47 ASC CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge