caa a22 4500 386321809 CHVBK 20180307111629.0 cr unu---uuuuu 161130e198801 xx s 000 0 eng 10.1079/BJN19880019 doi S0007114588000200 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880019 Determination of leucine metabolism and protein turnover in sheep, using gas-liquid chromatography-mass spectrometry [Elektronische Daten] 1. Whole-body protein synthetic rates in non-pregnant ewes were determined by the continuous infusion of L-[15N]- and [1-13C]leucine and measuring the plasma enrichment of leucine, α-ketoisocaproate (α-KIC) and expired carbon dioxide by gas-liquid chromatography-mass spectrometry. 2. The mean whole-body protein synthesis estimated from plasma leucine flux corrected for oxidation was 5·38 (SE 0·54) g/kg per d. 3. Under the conditions of the present study leucine oxidation was 0·323 (SE 0·067) mmol/kg per d and accounted for 10·71 (SE 2·26) % of plasma [13C]leucine flux. Deamination of leucine was 0·55 (SE 0·035) mmol/kg per d and accounted for approximately 17% of plasma [15N]leucine flux. 4. The rate of α-KIC reamination to leucine, calculated by subtracting 13C flux from 15N flux, was 0·228 (SE 0·101) mmol/kg per d. 5. The rate of whole-body protein degradation was 4·49 (SE 0·54) g/kg per d and there was a net protein gain of 0·89 (SE 0·21) g/kg per d. Copyright © The Nutrition Society 1988 Krishnamurti C. R. Department of Animal Science, University of British Columbia, Vancouver, B.C. V6T 2A2, Canada Janssens S. M. Department of Animal Science, University of British Columbia, Vancouver, B.C. V6T 2A2, Canada British Journal of Nutrition Cambridge University Press 59/1(1988-01), 155-164 0007-1145 59:1<155 1988 59 BJN https://doi.org/10.1079/BJN19880019 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krishnamurti C. R. Department of Animal Science, University of British Columbia, Vancouver, B.C. V6T 2A2, Canada NATIONALLICENCE 700 1- Janssens S. M. Department of Animal Science, University of British Columbia, Vancouver, B.C. V6T 2A2, Canada NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 59/1(1988-01), 155-164 0007-1145 59:1<155 1988 59 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge