caa a22 4500 386322007 CHVBK 20180307111629.0 cr unu---uuuuu 161130e198807 xx s 000 0 eng 10.1079/BJN19880075 doi S0007114588000765 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880075 The effect of Intralipid® infusion on the human leucocyte sodium-pump in vivo [Elektronische Daten] 1. The effect of unsaturated long-chain non-esterified fatty acids (NEFA) on the human leucocyte sodium-pump was studied in vivo. 2. Plasma NEFA level was raised acutely from 0·28 (sd 0·10) to 2·54 (sd 0·48) mmol/l by infusion of ‘Intralipid 20%' (trademark) at 90 ml/h with heparin, and the human leucocyte 22Na efflux rate constants were studied in eight normal weight males. 3. After 3 h, there was a significant lowering of the total (from 3·97 (sd 0·92) to 3·10 (sd 0·71)/h; P < 0·01) and ouabain-sensitive 22Na efflux rate constants (from 2·89 (sd 0·55) to 2·37 (sd 0·62)/h; P < 0·02). Ouabain-insensitive efflux rate constants showed a tendency to fall (from 1·08 (sd 0·51) to 0·73 (sd 0·23)/h). Leucocyte potassium content remained unchanged, but sodium content rose from 31 (sd 12) to 38 (sd 18) mmol/kg dry weight (P < 0·05). Total, ouabain-insensitive and ouabain-sensitive efflux rates did not change significantly during the Intralipid-heparin infusion. 4. Plasma insulin levels rose gradually throughout the 3 h infusion period. 5. In conclusion, NEFA, when raised to pathological levels, can inhibit the leucocyte Na-pump in vivo even in the presence of physiological levels of serum albumin, and may increase insulin secretion. Copyright © The Nutrition Society 1988 Ng L. L. Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE Hockaday T. D. R. Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE British Journal of Nutrition Cambridge University Press 60/1(1988-07), 49-55 0007-1145 60:1<49 1988 60 BJN https://doi.org/10.1079/BJN19880075 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880075 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ng L. L. Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE NATIONALLICENCE 700 1- Hockaday T. D. R. Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/1(1988-07), 49-55 0007-1145 60:1<49 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge