caa a22 4500 386322015 CHVBK 20180307111629.0 cr unu---uuuuu 161130e198807 xx s 000 0 eng 10.1079/BJN19880085 doi S0007114588000868 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880085 Initial studies on leucine metabolism in the rumen of sheep [Elektronische Daten] 1. [3H]leucine infused directly into the rumen of sheep labelled microbial protein and, when compared with the specific activity of the rumen free-leucine pool, indicated that 50% of the bacterial protein leucine originated from the rumen free-leucine pool. 2. The lower limit for bacterial protein turnover in the rumen was 0.37/d when calculated as the difference between the specific rate of disappearances of labelled bacteria from the rumen and the liquid-phase dilution rate. 3. Intravenously infused leucine also labelled the rumen bacteria. The build-up of specific activity in the rumen bacteria was sigmoidal and did not resemble that of the salivary protein which suggested that the rumen epithelium was a major endogenous protein input to the rumen. Additionally, bacteria isolated from the rumen epithelium had high radioactivity indicating that they were ingesting the rumen epithelial cells. Copyright © The Nutrition Society 1988 Marsden M. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD Bruce C. I. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD Bartram C. G. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD Buttery P. J. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD British Journal of Nutrition Cambridge University Press 60/1(1988-07), 161-171 0007-1145 60:1<161 1988 60 BJN https://doi.org/10.1079/BJN19880085 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880085 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marsden M. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD NATIONALLICENCE 700 1- Bruce C. I. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD NATIONALLICENCE 700 1- Bartram C. G. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD NATIONALLICENCE 700 1- Buttery P. J. Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leics. LE12 5RD NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/1(1988-07), 161-171 0007-1145 60:1<161 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge