caa a22 4500 386322090 CHVBK 20180307111630.0 cr unu---uuuuu 161130e198807 xx s 000 0 eng 10.1079/BJN19880071 doi S0007114588000728 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880071 Uptake of exogenous and endogenous eicosapentaenoic acid by cultured human mononuclear cells [Elektronische Daten] 1. Peripheral blood mononuclear cells from eight healthy volunteers were cultured, with or without concanavalin A (Con A), in a medium containing (ml/1) 100 normal autologous serum, 100 experimental autologous serum or 100 heterologous (fetal calf) serum. 2. The control and experimental autologous sera were obtained from the volunteers, before and after 15 d supplementation with 15 g fish oil (MaxEPA)/d to provide 1.5 g eicosapentaenoic acid (EPA; 20:5n-3)/d. The sera were frozen at -20°. The level of EPA increased from trace quantities in the control autologous serum to 14.3% w/w free fatty acids and between 6.9 and 8.1% w/w lipoprotein phospholipids in the experimental autologous serum. The heterologous fetal calf serum was enriched with EPA, complexed with bovine serum albumin, to provide a final concentration of 15μg/ml. All culture medium contained 10 ml fresh autologous serum/l and cells were obtained from the volunteers for the culture studies about 60 d after the end of EPA supplementation. 3. Portions of cells were removed from culture at 36, 48 and 72 h for phospholipid fatty acid analysis. 4. The level of EPA in phospholipids of cells cultured with exogenous EPA in fetal calf serum was increased significantly (P < 0.05) at all sampling times, both with and without Con A. By 48 h the levels had peaked at 15.8 (se 2.7) and 18.4 (se 4.5) % w/w respectively. 5. Resting cells, i.e. with no Con A present, failed to accumulate EPA when cultured in the experimental autologous serum containing 8.6% w/w total lipids as endogenous EPA. When cells were stimulated to divide by the mitogen Con A, there was a gradual increase in phospholipid EPA levels of mononuclear cells cultured with experimental autologous serum containing endogenous EPA. The maximum level obtained was at 72 h (7.2 (se 2.7)). 6. In a separate experiment, the effect of Con A at increasing doses (0, 1,5, 10 and 20 μg, final concentration) on lymphocyte blastogenesis was determined by estimating the incorporation of 3[H]thymidine. No significant effects due to serum in the culture medium were observed. Copyright © The Nutrition Society 1988 Gibney M. J. Division of Nutritional Sciences, Department of Clinical Medicine, Trinity College Medical School, St. James's Hospital, Dublin 8, Irish Republic Connolly Aedeen Division of Nutritional Sciences, Department of Clinical Medicine, Trinity College Medical School, St. James's Hospital, Dublin 8, Irish Republic British Journal of Nutrition Cambridge University Press 60/1(1988-07), 13-20 0007-1145 60:1<13 1988 60 BJN https://doi.org/10.1079/BJN19880071 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880071 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gibney M. J. Division of Nutritional Sciences, Department of Clinical Medicine, Trinity College Medical School, St. James's Hospital, Dublin 8, Irish Republic NATIONALLICENCE 700 1- Connolly Aedeen Division of Nutritional Sciences, Department of Clinical Medicine, Trinity College Medical School, St. James's Hospital, Dublin 8, Irish Republic NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/1(1988-07), 13-20 0007-1145 60:1<13 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge