caa a22 4500 386322198 CHVBK 20180307111630.0 cr unu---uuuuu 161130e198805 xx s 000 0 eng 10.1079/BJN19880059 doi S0007114588000613 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880059 Influence of goat's-milk folate-binding protein on transport of 5-methyltetrahydrofolate in neonatal-goat small intestinal brush-border-membrane vesicles [Elektronische Daten] 1. The influence of goat's-milk folate-binding protein (FBP) on the uptake of 5-methyltetrahydrofolate (MTHF) by brush-border-membrane vesicles prepared from the small intestine of the 6-d-old goat was investigated using a rapid-filtration assay. 2. Uptake of MTHF by the membrane vesicles was strongly enhanced by FBP within the pH range 4·5-6·5, with an optimum at pH 5-5·5. 3. Both the initial rate of MTHF uptake and uptake of MTHF at equilibrium were markedly increased in the presence of FBP. 4. Uptake of MTHF by brush-border-membrane vesicles was maximal when the molar ratio FBP: MTHF was 1·0-2·5. 5. The relation between pH and 125I-labelled FBP binding to the membranes was similar to that for uptake of MTHF, with an optimum at pH 5. 6. In experiments in which the osmotic pressure of the incubation medium was progressively increased with cellobiose, 125I-labelled FBP was found to be taken up primarily by binding to the brush-border-membrane surface. 7. Uptake of 125I-labelled FBP was time-dependent and saturable, with a Km of 0·39 (SE 0·07) μM and Vmax of 6·73 (SE 0·92) μg/mg protein. 8. Experiments in which various milk proteins (cow FBP, goat FBP, α-lactalbumin, β-lactoglobulin, bovine serum albumin and lactoferrin) were allowed to compete in turn with 125I-labelled FBP for uptake by brush-border-membrane vesicles indicated that high-affinity binding was probably specific to FBP, although lactoferrin reduced uptake possibly by non-specific coating of the mucosal surface. 9. It was concluded that a folate transport mechanism mediated by the FBP in milk exists at the intestinal brush border of neonatal goats. It is suggested that this may reinforce the developing endogenous transport system. Copyright © The Nutrition Society 1988 Salter Dallynn Department of Pig Nutrition and Production, AFRC Institute for Grassland and Animal Production, Shinfield, Reading RG2 9AQ Blakeborough Peter Department of Food Quality and Human Nutrition, AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT British Journal of Nutrition Cambridge University Press 59/3(1988-05), 497-507 0007-1145 59:3<497 1988 59 BJN https://doi.org/10.1079/BJN19880059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salter Dallynn Department of Pig Nutrition and Production, AFRC Institute for Grassland and Animal Production, Shinfield, Reading RG2 9AQ NATIONALLICENCE 700 1- Blakeborough Peter Department of Food Quality and Human Nutrition, AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 59/3(1988-05), 497-507 0007-1145 59:3<497 1988 59 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge