caa a22 4500 386322333 CHVBK 20180307111631.0 cr unu---uuuuu 161130e198805 xx s 000 0 eng 10.1079/BJN19880050 doi S0007114588000522 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880050 Influence of prostaglandins on unidirectional zinc fluxes across the small intestine of the rat [Elektronische Daten] 1. The regulatory role of prostaglandins (PGs) E2 and F2a on the zinc transport rate across the jejunal segments of rats was examined by employing the Ussing chamber technique. The Zn flux rate from mucosa to serosa across jejunal segments (Jms) was 5·24 (SE 1·54) nmol/h per cm2 (n 48) and that from serosa to mucosa (Jsm) was 15·16 (SE 2·38) nmol/h per cm2 (n 48) when both sides of the segment were bathed with Ringer's bicarbonate solution containing 0·5 mM-zinc chloride and 3 mM-L-histidine. 2. When 5·0 or 50 μM of either PGE2 or PGF2α were added to the serosal side of the tissue, Jsm generally decreased and Jms generally increased, compared with controls. On the other hand, when PGE2 or PGF2α was added to the mucosal side of the tissue, Jms either did not change or increased while Jsm had a tendency to decrease. 3. The Zn uptake capacity of tissue increased significantly when PG was added to the serosal side of the tissuebathing medium, but not when PG was added to the mucosal side. The uptake capacity of mucosal Zn by jejunal segments was approximately twice that of serosal Zn. 4. When PG was included in the tissue-bathing medium, the short-circuit current, potential difference and conductance between the mucosa- and serosa-bathing media generally decreased. 5. These results suggest that (a) PGs influence Zn flux rate not by chelating Zn and carrying it across the mucosal cell membrane but by interacting with the cytosolic components, (b) it is the serosal PGs which control the Zn flux rate and (c) PGs play a part in triggering a transduction mechanism in the intestinal Zn transport process. Copyright © The Nutrition Society 1988 Song Moon K. Research, Veterans Administration Medical Center, Sepulveda, CA 91343, USA Lee David B. N. Department of Pediatrics, University of California School of Medicine, Los Angeles, CA 90024, USA Adham Nabeel F. Department of Pediatrics, University of California School of Medicine, Los Angeles, CA 90024, USA British Journal of Nutrition Cambridge University Press 59/3(1988-05), 417-428 0007-1145 59:3<417 1988 59 BJN https://doi.org/10.1079/BJN19880050 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880050 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Moon K. Research, Veterans Administration Medical Center, Sepulveda, CA 91343, USA NATIONALLICENCE 700 1- Lee David B. N. Department of Pediatrics, University of California School of Medicine, Los Angeles, CA 90024, USA NATIONALLICENCE 700 1- Adham Nabeel F. Department of Pediatrics, University of California School of Medicine, Los Angeles, CA 90024, USA NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 59/3(1988-05), 417-428 0007-1145 59:3<417 1988 59 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge