caa a22 4500 386322406 CHVBK 20180307111631.0 cr unu---uuuuu 161130e198805 xx s 000 0 eng 10.1079/BJN19880057 doi S0007114588000595 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880057 Influence of dietary leucine content on the activities of branched-chain amino acid aminotransferase (EC 2.6.1.42) and branched-chain α-keto acid dehydrogenase (EC 1.2.4.4) complex in tissues of preruminant lambs [Elektronische Daten] 1. Branched-chain amino acid aminotransferase (EC 2.6.1.42; BCAAT) and branched-chain α-keto acid dehydrogenase (EC 1.2.4.4; BCKDH) activities were measured in preruminant lamb liver, longissimus dorsi muscle, kidney, jejunum and adipose tissue, 2 h after a meal with or without an excess of leucine. 2. Skeletal muscle contained about 70% of the total basal BCAAT activities of the tissues studied whereas liver contained about 60% of the total BCKDH activities of these tissues. 3. BCAAT activities were very low in preruminant lamb tissues. BCKDH was more phosphorylated in tissues of preruminant lambs than in rats, especially in liver. These low catalytic potentialities might contribute to a low rate of branched-chain amino acid catabolism in sheep. 4. Ingestion of an excess of leucine led to an increase in liver and jejunum BCAAT activities and activation of BCKDH in jejunum. Copyright © The Nutrition Society 1988 Papet Isabelle Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France Lezebot Nadia Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France Barre Francoise Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France Arnal Maurice Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France Harper Alfred E. Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA British Journal of Nutrition Cambridge University Press 59/3(1988-05), 475-483 0007-1145 59:3<475 1988 59 BJN https://doi.org/10.1079/BJN19880057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Papet Isabelle Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France NATIONALLICENCE 700 1- Lezebot Nadia Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France NATIONALLICENCE 700 1- Barre Francoise Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France NATIONALLICENCE 700 1- Arnal Maurice Laboratoire d'Etude du Métabolisme Azoté-INRA Theix, 63122 Ceyrat, France NATIONALLICENCE 700 1- Harper Alfred E. Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 59/3(1988-05), 475-483 0007-1145 59:3<475 1988 59 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge