caa a22 4500 386322503 CHVBK 20180307111631.0 cr unu---uuuuu 161130e198809 xx s 000 0 eng 10.1079/BJN19880096 doi S0007114588000972 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880096 Monitoring the fate of dietary proteins in rumen fluid using gel electrophoresis [Elektronische Daten] 1. When fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), strained rumen fluid from sheep fed on pelleted lucerne (Medicago sativa) hay showed no major protein components that stain with Coomassie Blue. This feature made it possible to monitor the fate of individual polypeptides within a protein mixture incubated in rumen fluid in vitro. 2. Extracts from a number of seed meals (sunflower (Helianthus annuus), lupin (Lupinus angustifolius), rape (Brassica napus) and pea (Pisum sativum L.)), as well as casein and bovine serum albumin, were examined in this system. The protein components of each seed type showed a wide range of resistances to degradation. One protein in pea seeds (pea albumin 1), which is particularly rich in cysteine, was almost as resistant to rumen degradation as bovine serum albumin. 3. Analysis of synthetic-fibre-bag experiments by SDS-PAGE showed that the rate of loss of total protein from solid meal residues does not provide an index of the resistance of individual protein components of the meal to rumen degradation. While there was no qualitative change in the protein profile of residual pea-seed meal inside a synthetic-fibre bag, there was considerable variation in the rate at which individual, solubilized protein components were degraded in the surrounding rumen fluid. Copyright © The Nutrition Society 1988 Spencer D. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia Higgins T. J. V. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia Freer M. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia Dove H. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia Coombe J. B. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia British Journal of Nutrition Cambridge University Press 60/2(1988-09), 241-247 0007-1145 60:2<241 1988 60 BJN https://doi.org/10.1079/BJN19880096 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880096 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Spencer D. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia NATIONALLICENCE 700 1- Higgins T. J. V. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia NATIONALLICENCE 700 1- Freer M. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia NATIONALLICENCE 700 1- Dove H. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia NATIONALLICENCE 700 1- Coombe J. B. Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, Australia NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/2(1988-09), 241-247 0007-1145 60:2<241 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge